論文 - 詳細
| RRC ID | 46303 |
|---|---|
| 著者 | Cox-Paulson EA, Grana TM, Harris MA, Batzli JM. |
| タイトル | Studying human disease genes in Caenorhabditis elegans: a molecular genetics laboratory project. |
| ジャーナル | CBE Life Sci Educ |
| Abstract |
Scientists routinely integrate information from various channels to explore topics under study. We designed a 4-wk undergraduate laboratory module that used a multifaceted approach to study a question in molecular genetics. Specifically, students investigated whether Caenorhabditis elegans can be a useful model system for studying genes associated with human disease. In a large-enrollment, sophomore-level laboratory course, groups of three to four students were assigned a gene associated with either breast cancer (brc-1), Wilson disease (cua-1), ovarian dysgenesis (fshr-1), or colon cancer (mlh-1). Students compared observable phenotypes of wild-type C. elegans and C. elegans with a homozygous deletion in the assigned gene. They confirmed the genetic deletion with nested polymerase chain reaction and performed a bioinformatics analysis to predict how the deletion would affect the encoded mRNA and protein. Students also performed RNA interference (RNAi) against their assigned gene and evaluated whether RNAi caused a phenotype similar to that of the genetic deletion. As a capstone activity, students prepared scientific posters in which they presented their data, evaluated whether C. elegans was a useful model system for studying their assigned genes, and proposed future directions. Assessment showed gains in understanding genotype versus phenotype, RNAi, common bioinformatics tools, and the utility of model organisms. |
| 巻・号 | 11(2) |
| ページ | 165-79 |
| 公開日 | 2012-7-1 |
| DOI | 10.1187/cbe-11-06-0045 |
| PII | 11/2/165 |
| PMID | 22665589 |
| PMC | PMC3366902 |
| MeSH | Animals Caenorhabditis elegans / genetics* Comprehension Computational Biology Curriculum* Disease / genetics* Educational Measurement Female Gene Deletion Genes, Helminth / genetics* Genotype Humans Knowledge Laboratories* Male Mental Competency Models, Animal Molecular Biology / education* Phenotype Polymerase Chain Reaction RNA Interference Self Report Students |
| IF | 2.241 |
| 引用数 | 9 |
| WOS 分野 | EDUCATION, SCIENTIFIC DISCIPLINES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1145 tm678 |