RRC ID 46305
Author Craig AL, Moser SC, Bailly AP, Gartner A.
Title Methods for studying the DNA damage response in the Caenorhabdatis elegans germ line.
Journal Methods Cell Biol.
Abstract In response to genotoxic insults, cells activate DNA damage response pathways that either stimulate transient cell cycle arrest and DNA repair or induce apoptosis. The Caenorhabditis elegans germ line is now well established as a model system to study these processes in a genetically tractable, multicellular organism. Upon treatment with genotoxic agents, premeiotic C. elegans germ cells transiently halt cell cycle progression, whereas meiotic prophase germ cells in the late-pachytene stage undergo apoptosis. Further, accumulation of unrepaired meiotic recombination intermediates can also lead to apoptosis of affected pachytene cells. DNA damage-induced cell death requires key components of the evolutionarily conserved apoptotic machinery. Moreover, both cell cycle arrest and pachytene apoptosis responses depend on conserved DNA damage checkpoint proteins. Genetics- and genomics-based approaches that have demonstrated roles for conserved checkpoint proteins have also begun to uncover novel components of these response pathways. In this chapter, we briefly review the C. elegans DNA damage response field, discuss in detail methods currently used to assay DNA damage responses in C. elegans, and describe the development of new experimental tools that will facilitate a more comprehensive understanding of the DNA damage response.
Volume 107
Pages 321-52
Published 2012
DOI 10.1016/B978-0-12-394620-1.00011-4
PII B978-0-12-394620-1.00011-4
PMID 22226529
MeSH Animals Apoptosis / drug effects Apoptosis / radiation effects Biological Assay* Biomarkers / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Damage DNA Repair* Gamma Rays Germ Cells / drug effects Germ Cells / metabolism* Germ Cells / radiation effects Hydroxyurea / pharmacology Larva / drug effects Larva / metabolism* Larva / radiation effects Meiosis / drug effects Meiosis / genetics Meiosis / radiation effects Mitosis / drug effects Mitosis / genetics Mitosis / radiation effects RNA Interference Signal Transduction / genetics
IF 1.588
Times Cited 25
C.elegans tm2940