RRC ID 4632
Author Kouzaki H, Kidou S, Miura H, Kato K.
Title Genomic diversity of germinating scutellum specific gene P23k in barley and wheat.
Journal Genetica
Abstract P23k is a 23 kDa protein involved in sugar translocation in the scutellum of germinating barley seeds. The present study was carried out to provide the genomic characterization for P23k gene in terms of copy number, chromosome mapping, genetic mapping and expression analysis in germinating sculletum in two major Triticeae crops, barley and wheat, and their relatives. Southern blotting showed that a variable copy number with different restriction fragment sizes was found among 15 Hordeum accessions, while low copy number were found to be conserved in 23 Triticum and 3 Aegilops accessions. Genetic and physical mapping study identified that the P23k gene is duplicated in wild and cultivated barley on chromosomes 1H, 2H, and 3H, and further tandem duplication on chromosomes 1H and 3H. In contrast, the wheat P23k is located on chromosome 3A of durum wheat and at the distal portion of the long arms of 3A and 3D chromosomes of bread wheat. Northern blotting showed remarkably high accumulation of P23k transcript in the germinating scutellum in cultivated and wild barley, whereas very few or no accumulation was detected in diploid, tetraploid, and hexaploid wheat accessions. The present study suggests a simple scenario where the ancestral P23k is encoded on the distal portion of an ancestral chromosome of homoeologous chromosome 3. Beside of polyploidy, dispersed and tandem duplications could trigger generation of the P23k family in the Hordeum lineage, while an ancestral P23k has been conserved in homoeologous 3A and 3D chromosomes in the wheat lineage.
Volume 137(2)
Pages 233-42
Published 2009-11
DOI 10.1007/s10709-009-9394-7
PMID 19641998
MeSH Blotting, Northern Blotting, Southern Chromosome Mapping Evolution, Molecular* Gene Expression Profiling Genes, Duplicate / genetics Genetic Variation* Genome, Plant / genetics* Hordeum / genetics* Plant Proteins / genetics Ploidies* Seeds / chemistry Species Specificity Triticum / genetics*
IF 1.229
Times Cited 0
WOS Category GENETICS & HEREDITY
Resource
Barley OUH602
Wheat KT001-001 KT001-002 KT001-003 KT003-001 KT003-002 KT003-003 KT115-001 KT115-006 KT115-007 KT120-001 KT120-002 KT120-003 KT006-001 KT006-002 KT006-003 KT007-001 KT007-003 KT007-004 KT009-001 KT009-002 KT009-003 KT018-001 KT018-002 KT019-001 KT019-002