RRC ID 46324
著者 Rosu S, Libuda DE, Villeneuve AM.
タイトル Robust crossover assurance and regulated interhomolog access maintain meiotic crossover number.
ジャーナル Science
Abstract Most organisms rely on interhomolog crossovers (COs) to ensure proper meiotic chromosome segregation but make few COs per chromosome pair. By monitoring repair events at a defined double-strand break (DSB) site during Caenorhabditis elegans meiosis, we reveal mechanisms that ensure formation of the obligate CO while limiting CO number. We find that CO is the preferred DSB repair outcome in the absence of inhibitory effects of other (nascent) recombination events. Thus, a single DSB per chromosome pair is largely sufficient to ensure CO formation. Further, we show that access to the homolog as a repair template is regulated, shutting down simultaneously for both CO and noncrossover (NCO) pathways. We propose that regulation of interhomolog access limits CO number and contributes to CO interference.
巻・号 334(6060)
ページ 1286-9
公開日 2011-12-2
DOI 10.1126/science.1212424
PII 334/6060/1286
PMID 22144627
PMC PMC3360972
MeSH Animals Caenorhabditis elegans / genetics* Chromosome Pairing Crossing Over, Genetic* DNA Breaks, Double-Stranded DNA Repair DNA, Helminth / genetics* Meiosis* Recombination, Genetic
IF 41.846
引用数 73
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1866