論文 - 詳細
| RRC ID | 46344 |
|---|---|
| 著者 | Schultheis C, Brauner M, Liewald JF, Gottschalk A. |
| タイトル | Optogenetic analysis of GABAB receptor signaling in Caenorhabditis elegans motor neurons. |
| ジャーナル | J Neurophysiol |
| Abstract |
In the nervous system, a perfect balance of excitation and inhibition is required, for example, to enable coordinated locomotion. In Caenorhabditis elegans, cholinergic and GABAergic motor neurons (MNs) effect waves of contralateral muscle contraction and relaxation. Cholinergic MNs innervate muscle as well as GABAergic MNs, projecting to the opposite side of the body, at dyadic synapses. Only a few connections exist from GABAergic to cholinergic MNs, emphasizing that GABA signaling is mainly directed toward muscle. Yet, a GABA(B) receptor comprising GBB-1 and GBB-2 subunits, expressed in cholinergic MNs, was shown to affect locomotion, likely by feedback inhibition of cholinergic MNs in response to spillover GABA. In the present study, we examined whether the GBB-1/2 receptor could also affect short-term plasticity in cholinergic MNs with the use of channelrhodopsin-2-mediated photostimulation of GABAergic and cholinergic neurons. The GBB-1/2 receptor contributes to acute body relaxation, evoked by photoactivation of GABAergic MNs, and to effects of GABA on locomotion behavior. Loss of the plasma membrane GABA transporter SNF-11, as well as acute photoevoked GABA release, affected cholinergic MN function in opposite directions. Prolonged stimulation of GABA MNs had subtle effects on cholinergic MNs, depending on stimulus duration and gbb-2. Thus GBB-1/2 receptors serve mainly for linear feedback inhibition of cholinergic MNs but also evoke minor plastic changes. |
| 巻・号 | 106(2) |
| ページ | 817-27 |
| 公開日 | 2011-8-1 |
| DOI | 10.1152/jn.00578.2010 |
| PII | jn.00578.2010 |
| PMID | 21613582 |
| PMC | PMC3154801 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / physiology* Cells, Cultured Molecular Sequence Data Motor Activity / physiology Motor Neurons / physiology* Photic Stimulation / methods* Receptors, GABA-B / physiology* Signal Transduction / physiology* |
| IF | 2.234 |
| 引用数 | 15 |
| WOS 分野 | PHYSIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1165 tm1406 |