論文 - 詳細
| RRC ID | 46350 |
|---|---|
| 著者 | Hall SE, Chirn GW, Lau NC, Sengupta P. |
| タイトル | RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans. |
| ジャーナル | RNA |
| Abstract |
Early environmental experiences profoundly influence adult phenotypes through complex mechanisms that are poorly understood. We previously showed that adult Caenorhabditis elegans that transiently passed through the stress-induced dauer larval stage (post-dauer adults) exhibit significant changes in gene expression profiles, chromatin states, and life history traits when compared with adults that bypassed the dauer stage (control adults). These wild-type, isogenic animals of equivalent developmental stages exhibit different signatures of molecular marks that reflect their distinct developmental trajectories. To gain insight into the mechanisms that contribute to these developmental history-dependent phenotypes, we profiled small RNAs from post-dauer and control adults by deep sequencing. RNA interference (RNAi) pathways are known to regulate genome-wide gene expression both at the chromatin and post-transcriptional level. By quantifying changes in endogenous small interfering RNA (endo-siRNA) levels in post-dauer as compared with control animals, our analyses identified a subset of genes that are likely targets of developmental history-dependent reprogramming through a complex RNAi-mediated mechanism. Mutations in specific endo-siRNA pathways affect expected gene expression and chromatin state changes for a subset of genes in post-dauer animals, as well as disrupt their increased brood size phenotype. We also find that both chromatin state and endo-siRNA distribution in dauers are unique, and suggest that remodeling in dauers provides a template for the subsequent establishment of adult post-dauer profiles. Our results indicate a role for endo-siRNA pathways as a contributing mechanism to early experience-dependent phenotypic plasticity in adults, and describe how developmental history can program adult physiology and behavior via epigenetic mechanisms. |
| 巻・号 | 19(3) |
| ページ | 306-19 |
| 公開日 | 2013-3-1 |
| DOI | 10.1261/rna.036418.112 |
| PII | rna.036418.112 |
| PMID | 23329696 |
| PMC | PMC3677242 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Chromatin / genetics Gene Expression Profiling Gene Expression Regulation, Developmental Genome, Helminth Phenotype* RNA Interference* RNA, Small Interfering / metabolism Signal Transduction |
| IF | 4.32 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm892 tm894 tm914 tm110 tm2686 tm1414 tm1127 tm1113 tm1019 tm1200 tm1195 tm1186 tm1116 tm1860 |