| RRC ID |
46350
|
| 著者 |
Hall SE, Chirn GW, Lau NC, Sengupta P.
|
| タイトル |
RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans.
|
| ジャーナル |
RNA
|
| Abstract |
Early environmental experiences profoundly influence adult phenotypes through complex mechanisms that are poorly understood. We previously showed that adult Caenorhabditis elegans that transiently passed through the stress-induced dauer larval stage (post-dauer adults) exhibit significant changes in gene expression profiles, chromatin states, and life history traits when compared with adults that bypassed the dauer stage (control adults). These wild-type, isogenic animals of equivalent developmental stages exhibit different signatures of molecular marks that reflect their distinct developmental trajectories. To gain insight into the mechanisms that contribute to these developmental history-dependent phenotypes, we profiled small RNAs from post-dauer and control adults by deep sequencing. RNA interference (RNAi) pathways are known to regulate genome-wide gene expression both at the chromatin and post-transcriptional level. By quantifying changes in endogenous small interfering RNA (endo-siRNA) levels in post-dauer as compared with control animals, our analyses identified a subset of genes that are likely targets of developmental history-dependent reprogramming through a complex RNAi-mediated mechanism. Mutations in specific endo-siRNA pathways affect expected gene expression and chromatin state changes for a subset of genes in post-dauer animals, as well as disrupt their increased brood size phenotype. We also find that both chromatin state and endo-siRNA distribution in dauers are unique, and suggest that remodeling in dauers provides a template for the subsequent establishment of adult post-dauer profiles. Our results indicate a role for endo-siRNA pathways as a contributing mechanism to early experience-dependent phenotypic plasticity in adults, and describe how developmental history can program adult physiology and behavior via epigenetic mechanisms.
|
| 巻・号 |
19(3)
|
| ページ |
306-19
|
| 公開日 |
2013-3-1
|
| DOI |
10.1261/rna.036418.112
|
| PII |
rna.036418.112
|
| PMID |
23329696
|
| PMC |
PMC3677242
|
| MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Chromatin / genetics
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genome, Helminth
Phenotype*
RNA Interference*
RNA, Small Interfering / metabolism
Signal Transduction
|
| IF |
4.32
|
| 引用数 |
25
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm892
tm894
tm914
tm110
tm2686
tm1414
tm1127
tm1113
tm1019
tm1200
tm1195
tm1186
tm1116
tm1860 |
