論文 - 詳細
| RRC ID | 46365 |
|---|---|
| 著者 | Hsieh YW, Chang C, Chuang CF. |
| タイトル | The microRNA mir-71 inhibits calcium signaling by targeting the TIR-1/Sarm1 adaptor protein to control stochastic L/R neuronal asymmetry in C. elegans. |
| ジャーナル | PLoS Genet |
| Abstract |
The Caenorhabditis elegans left and right AWC olfactory neurons communicate to establish stochastic asymmetric identities, AWC(ON) and AWC(OFF), by inhibiting a calcium-mediated signaling pathway in the future AWC(ON) cell. NSY-4/claudin-like protein and NSY-5/innexin gap junction protein are the two parallel signals that antagonize the calcium signaling pathway to induce the AWC(ON) fate. However, it is not known how the calcium signaling pathway is downregulated by nsy-4 and nsy-5 in the AWC(ON) cell. Here we identify a microRNA, mir-71, that represses the TIR-1/Sarm1 adaptor protein in the calcium signaling pathway to promote the AWC(ON) identity. Similar to tir-1 loss-of-function mutants, overexpression of mir-71 generates two AWC(ON) neurons. tir-1 expression is downregulated through its 3' UTR in AWC(ON), in which mir-71 is expressed at a higher level than in AWC(OFF). In addition, mir-71 is sufficient to inhibit tir-1 expression in AWC through the mir-71 complementary site in the tir-1 3' UTR. Our genetic studies suggest that mir-71 acts downstream of nsy-4 and nsy-5 to promote the AWC(ON) identity in a cell autonomous manner. Furthermore, the stability of mature mir-71 is dependent on nsy-4 and nsy-5. Together, these results provide insight into the mechanism by which nsy-4 and nsy-5 inhibit calcium signaling to establish stochastic asymmetric AWC differentiation. |
| 巻・号 | 8(8) |
| ページ | e1002864 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pgen.1002864 |
| PII | PGENETICS-D-11-02660 |
| PMID | 22876200 |
| PMC | PMC3410857 |
| MeSH | 3' Untranslated Regions Animals Body Patterning / genetics Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Calcium Signaling / genetics* Cell Communication Cell Differentiation Connexins / genetics Connexins / metabolism Cytoskeletal Proteins / genetics* Cytoskeletal Proteins / metabolism Gene Expression Regulation, Developmental Genes, Reporter Green Fluorescent Proteins MicroRNAs / genetics* MicroRNAs / metabolism Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / embryology Olfactory Receptor Neurons / metabolism* RNA Stability Receptors, G-Protein-Coupled |
| IF | 5.175 |
| 引用数 | 36 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2180 tm3036 |