論文 - 詳細
| RRC ID | 46383 |
|---|---|
| 著者 | Kim S, Ishidate T, Sharma R, Soto MC, Conte D Jr, Mello CC, Shirayama M. |
| タイトル | Wnt and CDK-1 regulate cortical release of WRM-1/β-catenin to control cell division orientation in early Caenorhabditis elegans embryos. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
In early Caenorhabditis elegans embryos, the Wingless/int (Wnt)- and Src-signaling pathways function in parallel to induce both the division orientation of the endomesoderm (EMS) blastomere and the endoderm fate of the posterior EMS daughter cell, called E. Here, we show that, in addition to its role in endoderm specification, the β-catenin-related protein Worm armadillo 1 (WRM-1) also plays a role in controlling EMS division orientation. WRM-1 localizes to the cortex of cells in both embryos and larvae and is released from the cortex in a Wnt-responsive manner. We show that WRM-1 cortical release is disrupted in a hypomorphic cyclin-dependent protein kinase 1 (cdk-1) mutant and that WRM-1 lacking potential CDK-1 phosphoacceptor sites is retained at the cortex. In both cases, cortical WRM-1 interferes with EMS spindle rotation without affecting endoderm specification. Finally, we show that removal of WRM-1 from the cortex can restore WT division orientation, even when both Wnt- and Src-signaling pathways are compromised. Our findings are consistent with a model in which Wnt signaling and CDK-1 modify WRM-1 in a temporal and spatial manner to unmask an intrinsic polarity cue required for proper orientation of the EMS cell division axis. |
| 巻・号 | 110(10) |
| ページ | E918-27 |
| 公開日 | 2013-3-5 |
| DOI | 10.1073/pnas.1300769110 |
| PII | 1300769110 |
| PMID | 23431196 |
| PMC | PMC3593879 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Division / genetics Cell Division / physiology Cell Polarity / genetics Cell Polarity / physiology Cyclin-Dependent Kinases / metabolism* Cytoskeletal Proteins / metabolism* Genes, Helminth Models, Biological Molecular Sequence Data Mutation Prophase / genetics Prophase / physiology Sequence Homology, Amino Acid Signal Transduction Spindle Apparatus / metabolism Wnt Proteins / metabolism* Wnt Signaling Pathway beta Catenin / metabolism* src-Family Kinases / metabolism |
| IF | 9.412 |
| 引用数 | 20 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm514 |