RRC ID 46387
著者 Knight AJ, Johnson NM, Behm CA.
タイトル VHA-19 is essential in Caenorhabditis elegans oocytes for embryogenesis and is involved in trafficking in oocytes.
ジャーナル PLoS One
Abstract There is an urgent need to develop new drugs against parasitic nematodes, which are a significant burden on human health and agriculture. Information about the function of essential nematode-specific genes provides insight to key nematode-specific processes that could be targeted with drugs. We have characterized the function of a novel, nematode-specific Caenorhabditis elegans protein, VHA-19, and show that VHA-19 is essential in the germline and, specifically, the oocytes, for the completion of embryogenesis. VHA-19 is also involved in trafficking the oocyte receptor RME-2 to the oocyte plasma membrane and is essential for osmoregulation in the embryo, probably because VHA-19 is required for proper eggshell formation via exocytosis of cortical granules or other essential components of the eggshell. VHA-19 may also have a role in cytokinesis, either directly or as an indirect effect of its role in osmoregulation. Critically, VHA-19 is expressed in the excretory cell in both larvae and adults, suggesting that it may have a role in osmoregulation in C. elegans more generally, probably in trafficking or secretion pathways. This is the first time a role for VHA-19 has been described.
巻・号 7(7)
ページ e40317
公開日 2012-1-1
DOI 10.1371/journal.pone.0040317
PII PONE-D-12-07050
PMID 22768351
PMC PMC3388054
MeSH Animals Biological Transport / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / genetics Cell Membrane / metabolism Embryo, Nonmammalian / metabolism* Embryonic Development / physiology* Humans Oocytes / cytology Oocytes / metabolism* Water-Electrolyte Balance / physiology*
IF 2.74
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm2205