RRC ID 46426
Author Pushpa K, Kumar GA, Subramaniam K.
Title PUF-8 and TCER-1 are essential for normal levels of multiple mRNAs in the C. elegans germline.
Journal Development
Abstract PUF family proteins are well-conserved regulators of cell proliferation in different developmental processes. They regulate target mRNAs by promoting degradation or by influencing translation through interaction with the translation initiation machinery. Here we show that Caenorhabditis elegans PUF-8 functions redundantly with the nuclear protein TCER-1 in the post-transcriptional maintenance of at least six germline mRNAs. The levels of spliced mRNAs in the puf-8(-) tcer-1(-) double mutant are only 10-30% of the wild type, whereas the unspliced forms increase by ∼2- to 3-fold compared with the wild type. These two proteins colocalise at the inner nuclear periphery, and their absence leads to reduced germ cell proliferation and to sterility. A yeast two-hybrid screen of 31 components of the nuclear pore complex and mRNA processing machineries identified seven proteins involved in mRNA export as potential partners of PUF-8. One of these, the nuclear cap-binding protein NCBP-2, colocalises with PUF-8 in the nucleus. A 50 amino acid N-terminal domain of PUF-8 is essential for interaction with NCBP-2 and for PUF-8 to function redundantly with TCER-1. These results reveal two important unexpected aspects of PUF proteins: that, in addition to the C-terminal PUF domain, the N-terminal domain is crucial for PUF function, and that PUF proteins have a novel role in mRNA maintenance. We propose that PUF proteins, in addition to their known cytoplasmic roles, participate in nuclear processing and/or export of mRNAs.
Volume 140(6)
Pages 1312-20
Published 2013-3-1
DOI 10.1242/dev.087833
PII 140/6/1312
PMID 23444359
PMC PMC3585663
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation, Developmental Germ Cells / growth & development Germ Cells / metabolism* Peptide Elongation Factors / genetics Peptide Elongation Factors / metabolism Peptide Elongation Factors / physiology* Protein Binding RNA Processing, Post-Transcriptional / genetics RNA Transport / genetics RNA, Messenger / genetics* RNA, Messenger / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* Tissue Distribution Two-Hybrid System Techniques
IF 5.611
Times Cited 9
C.elegans tm1452 tm2782