RRC ID 46438
著者 Schumacher JA, Hsieh YW, Chen S, Pirri JK, Alkema MJ, Li WH, Chang C, Chuang CF.
タイトル Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans.
ジャーナル Development
Abstract The C. elegans left and right AWC olfactory neurons specify asymmetric subtypes, one default AWC(OFF) and one induced AWC(ON), through a stochastic, coordinated cell signaling event. Intercellular communication between AWCs and non-AWC neurons via a NSY-5 gap junction network coordinates AWC asymmetry. However, the nature of intercellular signaling across the network and how individual non-AWC cells in the network influence AWC asymmetry is not known. Here, we demonstrate that intercellular calcium signaling through the NSY-5 gap junction neural network coordinates a precise 1AWC(ON)/1AWC(OFF) decision. We show that NSY-5 gap junctions in C. elegans cells mediate small molecule passage. We expressed vertebrate calcium-buffer proteins in groups of cells in the network to reduce intracellular calcium levels, thereby disrupting intercellular communication. We find that calcium in non-AWC cells of the network promotes the AWC(ON) fate, in contrast to the autonomous role of calcium in AWCs to promote the AWC(OFF) fate. In addition, calcium in specific non-AWCs promotes AWC(ON) side biases through NSY-5 gap junctions. Our results suggest a novel model in which calcium has dual roles within the NSY-5 network: autonomously promoting AWC(OFF) and non-autonomously promoting AWC(ON).
巻・号 139(22)
ページ 4191-201
公開日 2012-11-1
DOI 10.1242/dev.083428
PII 139/22/4191
PMID 23093425
PMC PMC3478688
MeSH Animals Biological Transport Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Calbindins Calcium / metabolism Calcium Signaling* Cell Communication Cells, Cultured Connexins / metabolism* Gap Junctions / metabolism* Gene Expression Regulation, Developmental Ion Channels / metabolism Membrane Proteins / metabolism Neurons / cytology* Neurons / metabolism Olfactory Pathways Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / metabolism* S100 Calcium Binding Protein G / metabolism Signal Transduction
IF 5.611
引用数 30
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm1340