論文 - 詳細
| RRC ID | 46438 |
|---|---|
| 著者 | Schumacher JA, Hsieh YW, Chen S, Pirri JK, Alkema MJ, Li WH, Chang C, Chuang CF. |
| タイトル | Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans. |
| ジャーナル | Development |
| Abstract |
The C. elegans left and right AWC olfactory neurons specify asymmetric subtypes, one default AWC(OFF) and one induced AWC(ON), through a stochastic, coordinated cell signaling event. Intercellular communication between AWCs and non-AWC neurons via a NSY-5 gap junction network coordinates AWC asymmetry. However, the nature of intercellular signaling across the network and how individual non-AWC cells in the network influence AWC asymmetry is not known. Here, we demonstrate that intercellular calcium signaling through the NSY-5 gap junction neural network coordinates a precise 1AWC(ON)/1AWC(OFF) decision. We show that NSY-5 gap junctions in C. elegans cells mediate small molecule passage. We expressed vertebrate calcium-buffer proteins in groups of cells in the network to reduce intracellular calcium levels, thereby disrupting intercellular communication. We find that calcium in non-AWC cells of the network promotes the AWC(ON) fate, in contrast to the autonomous role of calcium in AWCs to promote the AWC(OFF) fate. In addition, calcium in specific non-AWCs promotes AWC(ON) side biases through NSY-5 gap junctions. Our results suggest a novel model in which calcium has dual roles within the NSY-5 network: autonomously promoting AWC(OFF) and non-autonomously promoting AWC(ON). |
| 巻・号 | 139(22) |
| ページ | 4191-201 |
| 公開日 | 2012-11-1 |
| DOI | 10.1242/dev.083428 |
| PII | 139/22/4191 |
| PMID | 23093425 |
| PMC | PMC3478688 |
| MeSH | Animals Biological Transport Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Calbindins Calcium / metabolism Calcium Signaling* Cell Communication Cells, Cultured Connexins / metabolism* Gap Junctions / metabolism* Gene Expression Regulation, Developmental Ion Channels / metabolism Membrane Proteins / metabolism Neurons / cytology* Neurons / metabolism Olfactory Pathways Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / metabolism* S100 Calcium Binding Protein G / metabolism Signal Transduction |
| IF | 5.611 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1340 |