論文 - 詳細
| RRC ID | 46441 |
|---|---|
| 著者 | Sengupta MS, Low WY, Patterson JR, Kim HM, Traven A, Beilharz TH, Colaiácovo MP, Schisa JA, Boag PR. |
| タイトル | ifet-1 is a broad-scale translational repressor required for normal P granule formation in C. elegans. |
| ジャーナル | J Cell Sci |
| Abstract |
Large cytoplasmic ribonucleoprotein germ granule complexes are a common feature in germ cells. In C. elegans these are called P granules and for much of the life-cycle they associate with nuclear pore complexes in germ cells. P granules are rich in proteins that function in diverse RNA pathways. Here we report that the C. elegans homolog of the eIF4E-transporter IFET-1 is required for oogenesis but not spermatogenesis. We show that IFET-1 is required for translational repression of several maternal mRNAs in the distal gonad and functions in conjunction with the broad-scale translational regulators CGH-1, CAR-1 and PATR-1 to regulate germ cell sex determination. Furthermore we have found that IFET-1 localizes to P granules throughout the gonad and in the germ cell lineage in the embryo. Interestingly, IFET-1 is required for the normal ultrastructure of P granules and for the localization of CGH-1 and CAR-1 to P granules. Our findings suggest that IFET-1 is a key translational regulator and is required for normal P granule formation. |
| 巻・号 | 126(Pt 3) |
| ページ | 850-9 |
| 公開日 | 2013-2-1 |
| DOI | 10.1242/jcs.119834 |
| PII | jcs.119834 |
| PMID | 23264733 |
| PMC | PMC3619813 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cells, Cultured Cytoplasmic Granules / metabolism* Eukaryotic Initiation Factor-4E / genetics Germ Cells / physiology* Mutation / genetics Nuclear Pore / metabolism* Oogenesis / genetics Protein Biosynthesis Protein Transport RNA Nucleotidyltransferases / metabolism RNA-Binding Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Sequence Homology, Amino Acid Sex Determination Processes |
| IF | 4.573 |
| 引用数 | 20 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm2944 |