RRC ID |
46445
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著者 |
Singaravelu G, Chatterjee I, Rahimi S, Druzhinina MK, Kang L, Xu XZ, Singson A.
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タイトル |
The sperm surface localization of the TRP-3/SPE-41 Ca2+ -permeable channel depends on SPE-38 function in Caenorhabditis elegans.
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ジャーナル |
Dev Biol
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Abstract |
Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-permeable channel. Localization of both of these proteins is confined to the membranous organelles (MOs) in undifferentiated spermatids. In mature spermatozoa, SPE-38 is localized to the pseudopod and TRP-3/SPE-41 is localized to the whole plasma membrane. Here we show that the dynamic redistribution of TRP-3/SPE-41 from MOs to the plasma membrane is dependent on SPE-38. In spe-38 mutant spermatozoa, TRP-3/SPE-41 is trapped within the MOs and fails to reach the cell surface despite MO fusion with the plasma membrane. Split-ubiquitin yeast-two-hybrid analyses revealed that the cell surface localization of TRP-3/SPE-41 is likely regulated by SPE-38 through a direct protein-protein interaction mechanism. We have identified sequences that influence the physical interaction between SPE-38 and TRP-3/SPE-41, and show that these sequences in SPE-38 are required for fertility in transgenic animals. Despite the mislocalization of TRP-3/SPE-41 in spe-38 mutant spermatozoa, ionomycin or thapsigargin induced influx of Ca(2+) remains unperturbed. This work reveals a new paradigm for the regulated surface localization of a Ca(2+)-permeable channel.
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巻・号 |
365(2)
|
ページ |
376-83
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公開日 |
2012-5-15
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DOI |
10.1016/j.ydbio.2012.02.037
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PII |
S0012-1606(12)00138-8
|
PMID |
22425620
|
PMC |
PMC3337337
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MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Calcium / metabolism
Calcium Channels / metabolism*
Cell Membrane / metabolism*
Male
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Organelles / metabolism
Protein Structure, Tertiary
Spermatozoa / metabolism*
|
IF |
2.896
|
引用数 |
12
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WOS 分野
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DEVELOPMENTAL BIOLOGY
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リソース情報 |
線虫 |
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