RRC ID 46447
Author Spiga FM, Prouteau M, Gotta M.
Title The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo.
Journal Dev Biol
Abstract Cell polarity is crucial for many aspects of cell and developmental biology. Cytoskeleton remodeling plays an essential role in the establishment of cell polarity. In the Caenorhabditis elegans one-cell embryo, while the actomyosin cytoskeleton is required for asymmetric localization of the PAR proteins, anterior PAR proteins exert a feedback regulation on contractility. Here we identify the TAO kinase KIN-18 as a regulator of cortical contractility in the early embryo. KIN-18 negatively regulates cortical contractions in a RHO-1 dependent manner and regulates RHO-1 cortical localization. KIN-18 contributes to polarity establishment by regulating the position of the boundary between anterior and posterior PAR proteins. Although KIN-18 is involved in polarity establishment, depletion of KIN-18 restores contractions in a par-3 mutant indicating that kin-18 is epistatic to par-3. We suggest a model in which KIN-18 provides a link between the cytoskeleton remodeling and polarity machineries, uncovering a role for TAO kinases in the regulation of cell polarity.
Volume 373(1)
Pages 26-38
Published 2013-1-1
DOI 10.1016/j.ydbio.2012.10.001
PII S0012-1606(12)00542-8
PMID 23064028
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Cell Cycle / genetics Cell Cycle / physiology* Cell Polarity / genetics Cell Polarity / physiology* Cytoskeleton / genetics Cytoskeleton / physiology* Epistasis, Genetic / genetics Fluorescent Antibody Technique Image Processing, Computer-Assisted Microscopy, Interference Protein Kinases / metabolism* Protein Kinases / physiology Protein Serine-Threonine Kinases RNA Interference Two-Hybrid System Techniques rho GTP-Binding Proteins / metabolism
IF 2.896
Times Cited 6
C.elegans tm853