論文 - 詳細
RRC ID | 46447 |
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著者 | Spiga FM, Prouteau M, Gotta M. |
タイトル | The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo. |
ジャーナル | Dev Biol |
Abstract |
Cell polarity is crucial for many aspects of cell and developmental biology. Cytoskeleton remodeling plays an essential role in the establishment of cell polarity. In the Caenorhabditis elegans one-cell embryo, while the actomyosin cytoskeleton is required for asymmetric localization of the PAR proteins, anterior PAR proteins exert a feedback regulation on contractility. Here we identify the TAO kinase KIN-18 as a regulator of cortical contractility in the early embryo. KIN-18 negatively regulates cortical contractions in a RHO-1 dependent manner and regulates RHO-1 cortical localization. KIN-18 contributes to polarity establishment by regulating the position of the boundary between anterior and posterior PAR proteins. Although KIN-18 is involved in polarity establishment, depletion of KIN-18 restores contractions in a par-3 mutant indicating that kin-18 is epistatic to par-3. We suggest a model in which KIN-18 provides a link between the cytoskeleton remodeling and polarity machineries, uncovering a role for TAO kinases in the regulation of cell polarity. |
巻・号 | 373(1) |
ページ | 26-38 |
公開日 | 2013-1-1 |
DOI | 10.1016/j.ydbio.2012.10.001 |
PII | S0012-1606(12)00542-8 |
PMID | 23064028 |
MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Cell Cycle / genetics Cell Cycle / physiology* Cell Polarity / genetics Cell Polarity / physiology* Cytoskeleton / genetics Cytoskeleton / physiology* Epistasis, Genetic / genetics Fluorescent Antibody Technique Image Processing, Computer-Assisted Microscopy, Interference Protein Kinases / metabolism* Protein Kinases / physiology Protein Serine-Threonine Kinases RNA Interference Two-Hybrid System Techniques rho GTP-Binding Proteins / metabolism |
IF | 2.896 |
引用数 | 6 |
WOS 分野 | DEVELOPMENTAL BIOLOGY |
リソース情報 | |
線虫 | tm853 |