Reference - Detail
| RRC ID | 46453 |
|---|---|
| Author | Takenaka M, Inoue H, Takeshima A, Kakura T, Hori T. |
| Title | C. elegans Rassf homolog, rasf-1, is functionally associated with rab-39 Rab GTPase in oxidative stress response. |
| Journal | Genes Cells |
| Abstract |
The Ras association domain family (Rassf) is one of the Ras effectors, which can bind to several GTP-charged Ras-like GTPases. The Rassf proteins are widely conserved beyond species from nematode to human. To explore the novel functions of Rassf proteins, we took advantage of nematode C. elegans as a model animal with only one Rassf homolog, T24F1.3 (rasf-1). The rasf-1-mutant as well as rasf-1-knockdown animals were found to be more sensitive to oxidative stress of arsenite than in wild type, indicating that rasf-1 is involved in oxidative stress response. We next screened for proteins that interact with RASF-1 by the yeast two-hybrid system and identified RAB-39 Rab GTPase as an interacting partner of RASF-1. We not only confirmed specific binding between these molecules but also demonstrated that RASF-1 binds to GTP-bound form but not GDP-bound form of RAB-39. Importantly, rab-39 mutant animals were also sensitive to oxidative stress, which was dependent on rasf-1 according to the epistasis analysis. Moreover, Rassf1 and Rab39, mammalian homologs of rasf-1 and rab-39, respectively, were shown to interact with each other in vitro. These results indicate that the RASF-1 functionally interacts with RAB-39 and that the interaction between their homologs is conserved in mammals. |
| Volume | 18(3) |
| Pages | 203-10 |
| Published | 2013-3-1 |
| DOI | 10.1111/gtc.12028 |
| PMID | 23294242 |
| MeSH | Animals Arsenites / toxicity Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Oxidative Stress* Protein Binding Two-Hybrid System Techniques rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* |
| IF | 1.655 |
| Times Cited | 5 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm5002 tm2466 |
| Yeast | |