RRC ID |
46459
|
著者 |
Uozumi T, Hirotsu T, Yoshida K, Yamada R, Suzuki A, Taniguchi G, Iino Y, Ishihara T.
|
タイトル |
Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.
|
ジャーナル |
Sci Rep
|
Abstract |
Responses to environmental stimuli are mediated by the activation and inactivation of various signalling proteins. However, the temporal dynamics of these events in living animals are not well understood. Here we show real-time imaging of the activity of the key regulator of the MAP kinase pathway, Ras, in living Caenorhabditis elegans and that Ras is transiently activated within a few seconds in olfactory neurons in response to increase in the concentration of odorants. This fast activation of Ras is dependent on the olfactory signalling pathway and Ras guanyl nucleotide-releasing protein (RasGRP). A negative feedback loop then quickly leads to Ras inactivation despite the continued presence of the odorant. Phenotypes of Ras mutants suggest this rapid activation and inactivation of Ras is important for regulation of interneuron activities and olfactory behaviours. Our results reveal novel kinetics and biological implication of transient activation of Ras in olfactory systems.
|
巻・号 |
2
|
ページ |
500
|
公開日 |
2012-1-1
|
DOI |
10.1038/srep00500
|
PII |
srep00500
|
PMID |
22787558
|
PMC |
PMC3391662
|
MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism
Feedback, Physiological
Genes, ras*
Guanylate Cyclase / metabolism
Interneurons / physiology
Membrane Proteins
Mitogen-Activated Protein Kinases / metabolism
Olfactory Pathways / physiology*
Olfactory Receptor Neurons / physiology
Protein Kinase C / metabolism
Signal Transduction
Transcriptional Activation*
|
IF |
3.998
|
引用数 |
11
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
線虫 |
tm2411 |