RRC ID 46459
著者 Uozumi T, Hirotsu T, Yoshida K, Yamada R, Suzuki A, Taniguchi G, Iino Y, Ishihara T.
タイトル Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.
ジャーナル Sci Rep
Abstract Responses to environmental stimuli are mediated by the activation and inactivation of various signalling proteins. However, the temporal dynamics of these events in living animals are not well understood. Here we show real-time imaging of the activity of the key regulator of the MAP kinase pathway, Ras, in living Caenorhabditis elegans and that Ras is transiently activated within a few seconds in olfactory neurons in response to increase in the concentration of odorants. This fast activation of Ras is dependent on the olfactory signalling pathway and Ras guanyl nucleotide-releasing protein (RasGRP). A negative feedback loop then quickly leads to Ras inactivation despite the continued presence of the odorant. Phenotypes of Ras mutants suggest this rapid activation and inactivation of Ras is important for regulation of interneuron activities and olfactory behaviours. Our results reveal novel kinetics and biological implication of transient activation of Ras in olfactory systems.
巻・号 2
ページ 500
公開日 2012-1-1
DOI 10.1038/srep00500
PII srep00500
PMID 22787558
PMC PMC3391662
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Feedback, Physiological Genes, ras* Guanylate Cyclase / metabolism Interneurons / physiology Membrane Proteins Mitogen-Activated Protein Kinases / metabolism Olfactory Pathways / physiology* Olfactory Receptor Neurons / physiology Protein Kinase C / metabolism Signal Transduction Transcriptional Activation*
IF 3.998
引用数 11
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm2411