Reference - Detail
| RRC ID | 46480 |
|---|---|
| Author | Zhang H, Kim A, Abraham N, Khan LA, Hall DH, Fleming JT, Gobel V. |
| Title | Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis. |
| Journal | Development |
| Abstract |
Clathrin coats vesicles in all eukaryotic cells and has a well-defined role in endocytosis, moving molecules away from the plasma membrane. Its function on routes towards the plasma membrane was only recently appreciated and is thought to be limited to basolateral transport. Here, an unbiased RNAi-based tubulogenesis screen identifies a role of clathrin (CHC-1) and its AP-1 adaptor in apical polarity during de novo lumenal membrane biogenesis in the C. elegans intestine. We show that CHC-1/AP-1-mediated polarized transport intersects with a sphingolipid-dependent apical sorting process. Depleting each presumed trafficking component mislocalizes the same set of apical membrane molecules basolaterally, including the polarity regulator PAR-6, and generates ectopic lateral lumens. GFP::CHC-1 and BODIPY-ceramide vesicles associate perinuclearly and assemble asymmetrically at polarized plasma membrane domains in a co-dependent and AP-1-dependent manner. Based on these findings, we propose a trafficking pathway for apical membrane polarity and lumen morphogenesis that implies: (1) a clathrin/AP-1 function on an apically directed transport route; and (2) the convergence of this route with a sphingolipid-dependent apical trafficking path. |
| Volume | 139(11) |
| Pages | 2071-83 |
| Published | 2012-6-1 |
| DOI | 10.1242/dev.077347 |
| PII | dev.077347 |
| PMID | 22535410 |
| PMC | PMC3347694 |
| MeSH | Adaptor Protein Complex 1 / metabolism Adaptor Protein Complex 1 / physiology* Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Polarity / physiology* Clathrin Heavy Chains / metabolism Clathrin Heavy Chains / physiology* Green Fluorescent Proteins Intestines / cytology Intestines / embryology* Microscopy, Electron, Transmission Microscopy, Fluorescence Protein Transport / physiology RNA Interference Sphingosine / analogs & derivatives Transport Vesicles / metabolism |
| IF | 5.611 |
| Times Cited | 33 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Blogs |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2866 tm935 tm1369 tm1425 |