| RRC ID |
46492
|
| Author |
Yoshina S, Sakaki K, Yonezumi-Hayashi A, Gengyo-Ando K, Inoue H, Iino Y, Mitani S.
|
| Title |
Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi.
|
| Journal |
Mol Biol Cell
|
| Abstract |
A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is required for gonad morphogenesis. ADAMTS9 and GON-1 have similar domain structures, and both have a unique C-terminal domain called the "GON domain," whose function remains unknown. Here we show that down-regulation of human ADAMTS9 and C. elegans GON-1 results in the inhibition of protein transport from the endoplasmic reticulum (ER) to the Golgi. This phenotype was rescued by the expression of the GON domain localizing in the ER in human cells and C. elegans. We propose a novel function of ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent.
|
| Volume |
23(9)
|
| Pages |
1728-41
|
| Published |
2012-5-1
|
| DOI |
10.1091/mbc.E11-10-0857
|
| PII |
mbc.E11-10-0857
|
| PMID |
22419820
|
| PMC |
PMC3338439
|
| MeSH |
ADAM Proteins / genetics
ADAM Proteins / metabolism*
ADAMTS9 Protein
Animals
Animals, Genetically Modified
Caenorhabditis elegans
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Line
Conserved Sequence
Endoplasmic Reticulum / metabolism*
Golgi Apparatus / metabolism*
HEK293 Cells
Humans
Metalloendopeptidases / genetics
Metalloendopeptidases / metabolism*
Protein Transport
|
| IF |
3.791
|
| Times Cited |
14
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm3146 |
