RRC ID 465
Author Ota A, Kusakabe T, Sugimoto Y, Takahashi M, Nakajima Y, Kawaguchi Y, Koga K.
Title Cloning and characterization of testis-specific tektin in Bombyx mori.
Journal Comp Biochem Physiol B Biochem Mol Biol
Abstract A testis-specific cDNA library of Bombyx mori was constructed by an mRNA subtraction technique. Several clones were randomly selected and determined for their nucleotide sequences. One of them, designated as BmTST, contained a 3'-part of an open reading frame homologous to tektin, the protein known to form filamentous polymers in the walls of ciliary and flagellar microtubules. Also isolated was a genomic fragment, which contains the 5'-part of the coding sequence of BmTST and its promoter region. As a whole, the complete open reading frame was found to encode 508 amino acid residues, whose sequence had 28, 28 and 30% identities with the Strongylocentrotus purpuratus tektins A1, B1 and C1, respectively. Expression analysis by reverse transcription polymerase chain reaction with the cDNA and Western blotting with a polyclonal antibody indicated that the BmTST gene was expressed specifically in the testis during sperm maturation. The protein was immunologically detected exclusively in the fraction expected to contain the 9 + 2 flagellar axonemes of sperms. We infer that the BmTst protein is possibly involved in the spermatogenesis of B. mori.
Volume 133(3)
Pages 371-82
Published 2002-11-1
DOI 10.1016/s1096-4959(02)00153-7
PII S1096495902001537
PMID 12431405
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Western Bombyx / genetics* Cloning, Molecular DNA, Complementary / genetics Gene Expression Profiling Gene Library Male Microtubule Proteins / analysis Microtubule Proteins / chemistry* Microtubule Proteins / genetics* Microtubules / chemistry Molecular Sequence Data Organ Specificity RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Alignment Sequence Homology, Amino Acid Spermatozoa / chemistry Testis / cytology Testis / metabolism*
IF 2.219
Times Cited 23
Silkworms r06 EST database