RRC ID 46512
著者 Sugioka K, Mizumoto K, Sawa H.
タイトル Wnt regulates spindle asymmetry to generate asymmetric nuclear β-catenin in C. elegans.
ジャーナル Cell
Abstract Extrinsic signals received by a cell can induce remodeling of the cytoskeleton, but the downstream effects of cytoskeletal changes on gene expression have not been well studied. Here, we show that during telophase of an asymmetric division in C. elegans, extrinsic Wnt signaling modulates spindle structures through APR-1/APC, which in turn promotes asymmetrical nuclear localization of WRM-1/β-catenin and POP-1/TCF. APR-1 that localized asymmetrically along the cortex established asymmetric distribution of astral microtubules, with more microtubules found on the anterior side. Perturbation of the Wnt signaling pathway altered this microtubule asymmetry and led to changes in nuclear WRM-1 asymmetry, gene expression, and cell-fate determination. Direct manipulation of spindle asymmetry by laser irradiation altered the asymmetric distribution of nuclear WRM-1. Moreover, laser manipulation of the spindles rescued defects in nuclear POP-1 asymmetry in wnt mutants. Our results reveal a mechanism in which the nuclear localization of proteins is regulated through the modulation of microtubules.
巻・号 146(6)
ページ 942-54
公開日 2011-9-16
DOI 10.1016/j.cell.2011.07.043
PII S0092-8674(11)00887-7
PMID 21925317
MeSH Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / metabolism Cytoskeletal Proteins / metabolism* Kinesins / metabolism Microtubules / metabolism Signal Transduction* Spindle Apparatus Wnt Proteins / metabolism* beta Catenin / metabolism
IF 38.637
引用数 50
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm2114 tm785 tm2143 tm375