論文 - 詳細
| RRC ID | 46559 |
|---|---|
| 著者 | Achilleos A, Wehman AM, Nance J. |
| タイトル | PAR-3 mediates the initial clustering and apical localization of junction and polarity proteins during C. elegans intestinal epithelial cell polarization. |
| ジャーナル | Development |
| Abstract |
The apicobasal polarity of epithelial cells is critical for organ morphogenesis and function, and loss of polarity can promote tumorigenesis. Most epithelial cells form when precursor cells receive a polarization cue, develop distinct apical and basolateral domains and assemble junctions near their apical surface. The scaffolding protein PAR-3 regulates epithelial cell polarity, but its cellular role in the transition from precursor cell to polarized epithelial cell has not been determined in vivo. Here, we use a targeted protein-degradation strategy to remove PAR-3 from C. elegans embryos and examine its cellular role as intestinal precursor cells become polarized epithelial cells. At initial stages of polarization, PAR-3 accumulates in cortical foci that contain E-cadherin, other adherens junction proteins, and the polarity proteins PAR-6 and PKC-3. Using live imaging, we show that PAR-3 foci move apically and cluster, and that PAR-3 is required to assemble E-cadherin into foci and for foci to accumulate at the apical surface. We propose that PAR-3 facilitates polarization by promoting the initial clustering of junction and polarity proteins that then travel and accumulate apically. Unexpectedly, superficial epidermal cells form apical junctions in the absence of PAR-3, and we show that PAR-6 has a PAR-3-independent role in these cells to promote apical junction maturation. These findings indicate that PAR-3 and PAR-6 function sequentially to position and mature apical junctions, and that the requirement for PAR-3 can vary in different types of epithelial cells. |
| 巻・号 | 137(11) |
| ページ | 1833-42 |
| 公開日 | 2010-6-1 |
| DOI | 10.1242/dev.047647 |
| PII | dev.047647 |
| PMID | 20431121 |
| PMC | PMC2867319 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Cadherins / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Polarity / physiology* DNA, Helminth / genetics Epithelial Cells / cytology Epithelial Cells / metabolism Gene Expression Regulation, Developmental Genes, Helminth Intercellular Junctions / metabolism Intestinal Mucosa / metabolism Intestines / cytology Intestines / embryology Molecular Sequence Data Mutation Protein Serine-Threonine Kinases RNA Interference |
| IF | 5.611 |
| 引用数 | 67 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2010 tm2716 tm1425 |