RRC ID 46565
Author Asikainen S, Rudgalvyte M, Heikkinen L, Louhiranta K, Lakso M, Wong G, Nass R.
Title Global microRNA expression profiling of Caenorhabditis elegans Parkinson's disease models.
Journal J Mol Neurosci
Abstract MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs that may be involved in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify potential gene expression changes in 115 annotated miRNAs in PD-associated Caenorhabditis elegans models that either overexpress human A53T alpha-synuclein or have mutations within the vesicular catecholamine transporter (cat-1) or parkin (pdr-1) ortholog. Here, we show that 12 specific miRNAs are differentially regulated in the animals overexpressing alpha-synuclein, five in cat-1, and three in the pdr-1 mutants. The family of miR-64 and miR-65 are co-underexpressed in the alpha-synuclein transgenic and cat-1 strains, and members of let-7 family co-underexpressed in the alpha-synuclein and pdr-1 strains; mdl-1 and ptc-1 genes are target candidates for miR-64 and miR-65 and are overexpressed in alpha-synuclein transgenic as well as miR-64/65 (tm3711) knockout animals. These results indicate that miRNAs are differentially expressed in C. elegans PD models and suggest a role for these molecules in disease pathogenesis.
Volume 41(1)
Pages 210-8
Published 2010-5-1
DOI 10.1007/s12031-009-9325-1
PMID 20091141
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Disease Models, Animal* Gene Expression Profiling Humans MicroRNAs* / genetics MicroRNAs* / metabolism Oligonucleotide Array Sequence Analysis / methods Parkinson Disease / genetics* Parkinson Disease / pathology Parkinson Disease / physiopathology
IF 2.678
Times Cited 70
C.elegans tm3711