RRC ID 46576
Author Chatzigeorgiou M, Yoo S, Watson JD, Lee WH, Spencer WC, Kindt KS, Hwang SW, Miller DM 3rd, Treinin M, Driscoll M, Schafer WR.
Title Specific roles for DEG/ENaC and TRP channels in touch and thermosensation in C. elegans nociceptors.
Journal Nat. Neurosci.
Abstract Polymodal nociceptors detect noxious stimuli, including harsh touch, toxic chemicals and extremes of heat and cold. The molecular mechanisms by which nociceptors are able to sense multiple qualitatively distinct stimuli are not well understood. We found that the C. elegans PVD neurons are mulitidendritic nociceptors that respond to harsh touch and cold temperatures. The harsh touch modality specifically required the DEG/ENaC proteins MEC-10 and DEGT-1, which represent putative components of a harsh touch mechanotransduction complex. In contrast, responses to cold required the TRPA-1 channel and were MEC-10 and DEGT-1 independent. Heterologous expression of C. elegans TRPA-1 conferred cold responsiveness to other C. elegans neurons and to mammalian cells, indicating that TRPA-1 is a cold sensor. Our results suggest that C. elegans nociceptors respond to thermal and mechanical stimuli using distinct sets of molecules and identify DEG/ENaC channels as potential receptors for mechanical pain.
Volume 13(7)
Pages 861-8
Published 2010-7
DOI 10.1038/nn.2581
PII nn.2581
PMID 20512132
PMC PMC2975101
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Epithelial Sodium Channels / metabolism Epithelial Sodium Channels / physiology* Mechanotransduction, Cellular / physiology* Membrane Proteins / metabolism Neurons / classification Nociceptors / physiology* Signal Transduction / physiology Sodium Channels / physiology* Thermosensing / physiology Touch / physiology Transient Receptor Potential Channels / metabolism
IF 21.126
Times Cited 81
WOS Category NEUROSCIENCES
Resource
C.elegans tm1552