RRC ID 46589
Author Flowers EB, Poole RJ, Tursun B, Bashllari E, Pe'er I, Hobert O.
Title The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans.
Journal Development
Abstract Transcriptional co-repressors of the Groucho/TLE family are important regulators of development in many species. A subset of Groucho/TLE family members that lack the C-terminal WD40 domains have been proposed to act as dominant-negative regulators of Groucho/TLE proteins, yet such a role has not been conclusively proven. Through a mutant screen for genes controlling a left/right asymmetric cell fate decision in the nervous system of the nematode C. elegans, we have retrieved loss-of-function alleles in two distinct loci that display identical phenotypes in neuronal fate specification and in other developmental contexts. Using the novel technology of whole-genome sequencing, we find that these loci encode the C. elegans ortholog of Groucho, UNC-37, and, surprisingly, a short Groucho-like protein, LSY-22, that is similar to truncated Groucho proteins in other species. Besides their phenotypic similarities, unc-37 and lsy-22 show genetic interactions and UNC-37 and LSY-22 proteins also physically bind to each other in vivo. Our findings suggest that rather than acting as negative regulators of Groucho, small Groucho-like proteins may promote Groucho function. We propose that Groucho-mediated gene regulatory events involve heteromeric complexes of distinct Groucho-like proteins.
Volume 137(11)
Pages 1799-805
Published 2010-6-1
DOI 10.1242/dev.046219
PII dev.046219
PMID 20431118
PMC PMC2867316
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Epistasis, Genetic Gene Expression Regulation, Developmental Models, Biological Molecular Sequence Data Mutation Neurogenesis / genetics Neurogenesis / physiology Repressor Proteins / chemistry Repressor Proteins / genetics* Repressor Proteins / metabolism* Sequence Homology, Amino Acid Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism* Two-Hybrid System Techniques
IF 5.763
Times Cited 24
C.elegans tm3750