論文 - 詳細
| RRC ID | 46589 |
|---|---|
| 著者 | Flowers EB, Poole RJ, Tursun B, Bashllari E, Pe'er I, Hobert O. |
| タイトル | The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. |
| ジャーナル | Development |
| Abstract |
Transcriptional co-repressors of the Groucho/TLE family are important regulators of development in many species. A subset of Groucho/TLE family members that lack the C-terminal WD40 domains have been proposed to act as dominant-negative regulators of Groucho/TLE proteins, yet such a role has not been conclusively proven. Through a mutant screen for genes controlling a left/right asymmetric cell fate decision in the nervous system of the nematode C. elegans, we have retrieved loss-of-function alleles in two distinct loci that display identical phenotypes in neuronal fate specification and in other developmental contexts. Using the novel technology of whole-genome sequencing, we find that these loci encode the C. elegans ortholog of Groucho, UNC-37, and, surprisingly, a short Groucho-like protein, LSY-22, that is similar to truncated Groucho proteins in other species. Besides their phenotypic similarities, unc-37 and lsy-22 show genetic interactions and UNC-37 and LSY-22 proteins also physically bind to each other in vivo. Our findings suggest that rather than acting as negative regulators of Groucho, small Groucho-like proteins may promote Groucho function. We propose that Groucho-mediated gene regulatory events involve heteromeric complexes of distinct Groucho-like proteins. |
| 巻・号 | 137(11) |
| ページ | 1799-805 |
| 公開日 | 2010-6-1 |
| DOI | 10.1242/dev.046219 |
| PII | dev.046219 |
| PMID | 20431118 |
| PMC | PMC2867316 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Epistasis, Genetic Gene Expression Regulation, Developmental Models, Biological Molecular Sequence Data Mutation Neurogenesis / genetics Neurogenesis / physiology Repressor Proteins / chemistry Repressor Proteins / genetics* Repressor Proteins / metabolism* Sequence Homology, Amino Acid Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism* Two-Hybrid System Techniques |
| IF | 5.611 |
| 引用数 | 24 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3750 |