RRC ID 46590
著者 Gallo CM, Wang JT, Motegi F, Seydoux G.
タイトル Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans.
ジャーナル Science
Abstract Asymmetric segregation of P granules during the first four divisions of the Caenorhabditis elegans embryo is a classic example of cytoplasmic partitioning of germline determinants. It is thought that asymmetric partitioning of P granule components during mitosis is essential to distinguish germline from soma. We have identified a mutant (pptr-1) in which P granules become unstable during mitosis and P granule proteins and RNAs are distributed equally to somatic and germline blastomeres. Despite symmetric partitioning of P granule components, pptr-1 mutants segregate a germline that uniquely expresses P granules during postembryonic development. pptr-1 mutants are fertile, except at high temperatures. Hence, asymmetric partitioning of maternal P granules is not essential to specify germ cell fate. Instead, it may serve to protect the nascent germline from stress.
巻・号 330(6011)
ページ 1685-9
公開日 2010-12-17
DOI 10.1126/science.1193697
PII science.1193697
PMID 21127218
PMC PMC3072820
MeSH Animals Blastomeres / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Cytoplasm / metabolism* Cytoplasmic Granules / physiology* Cytoplasmic Granules / ultrastructure Embryo, Nonmammalian / physiology Embryonic Development Germ Cells / physiology* Interphase Microscopy, Confocal Mitosis Mutation Nuclear Proteins / metabolism Protein Serine-Threonine Kinases / metabolism RNA, Helminth / metabolism* RNA-Binding Proteins / metabolism Recombinant Fusion Proteins / metabolism Zygote / physiology
IF 41.846
引用数 77
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm3103