Reference - Detail
| RRC ID | 46590 |
|---|---|
| Author | Gallo CM, Wang JT, Motegi F, Seydoux G. |
| Title | Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans. |
| Journal | Science |
| Abstract |
Asymmetric segregation of P granules during the first four divisions of the Caenorhabditis elegans embryo is a classic example of cytoplasmic partitioning of germline determinants. It is thought that asymmetric partitioning of P granule components during mitosis is essential to distinguish germline from soma. We have identified a mutant (pptr-1) in which P granules become unstable during mitosis and P granule proteins and RNAs are distributed equally to somatic and germline blastomeres. Despite symmetric partitioning of P granule components, pptr-1 mutants segregate a germline that uniquely expresses P granules during postembryonic development. pptr-1 mutants are fertile, except at high temperatures. Hence, asymmetric partitioning of maternal P granules is not essential to specify germ cell fate. Instead, it may serve to protect the nascent germline from stress. |
| Volume | 330(6011) |
| Pages | 1685-9 |
| Published | 2010-12-17 |
| DOI | 10.1126/science.1193697 |
| PII | science.1193697 |
| PMID | 21127218 |
| PMC | PMC3072820 |
| MeSH | Animals Blastomeres / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Cytoplasm / metabolism* Cytoplasmic Granules / physiology* Cytoplasmic Granules / ultrastructure Embryo, Nonmammalian / physiology Embryonic Development Germ Cells / physiology* Interphase Microscopy, Confocal Mitosis Mutation Nuclear Proteins / metabolism Protein Serine-Threonine Kinases / metabolism RNA, Helminth / metabolism* RNA-Binding Proteins / metabolism Recombinant Fusion Proteins / metabolism Zygote / physiology |
| IF | 41.846 |
| Times Cited | 77 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| C.elegans | tm3103 |