RRC ID 46592
Author Galvin BD, Denning DP, Horvitz HR.
Title SPK-1, an SR protein kinase, inhibits programmed cell death in Caenorhabditis elegans.
Journal Proc Natl Acad Sci U S A
Abstract To identify genes involved in protecting cells from programmed cell death in Caenorhabditis elegans, we performed a genetic screen to isolate mutations that cause an increase in the number of programmed cell deaths. We screened for suppressors of the cell-death defect caused by a partial loss-of-function mutation in ced-4, which encodes an Apaf-1 homolog that promotes programmed cell death by activating the caspase CED-3. We identified one extragenic ced-4 suppressor, which has a mutation in the gene spk-1. The spk-1 gene encodes a protein homologous to serine-arginine-rich (SR) protein kinases, which are thought to regulate splicing. Previous work suggests that ced-4 can be alternatively spliced and that the splice variants function oppositely, with the longer transcript (ced-4L) inhibiting programmed cell death. spk-1 might promote cell survival by increasing the amount of the protective ced-4L splice variant. We conclude that programmed cell death in C. elegans is regulated by an alternative splicing event controlled by the SR protein kinase SPK-1.
Volume 108(5)
Pages 1998-2003
Published 2011-2-1
DOI 10.1073/pnas.1018805108
PII 1018805108
PMID 21245325
PMC PMC3033281
MeSH Alleles Alternative Splicing Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcium-Binding Proteins / genetics Genes, Helminth Models, Genetic Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Suppression, Genetic
IF 9.412
Times Cited 8
C.elegans tm837 tm722