RRC ID |
46592
|
著者 |
Galvin BD, Denning DP, Horvitz HR.
|
タイトル |
SPK-1, an SR protein kinase, inhibits programmed cell death in Caenorhabditis elegans.
|
ジャーナル |
Proc Natl Acad Sci U S A
|
Abstract |
To identify genes involved in protecting cells from programmed cell death in Caenorhabditis elegans, we performed a genetic screen to isolate mutations that cause an increase in the number of programmed cell deaths. We screened for suppressors of the cell-death defect caused by a partial loss-of-function mutation in ced-4, which encodes an Apaf-1 homolog that promotes programmed cell death by activating the caspase CED-3. We identified one extragenic ced-4 suppressor, which has a mutation in the gene spk-1. The spk-1 gene encodes a protein homologous to serine-arginine-rich (SR) protein kinases, which are thought to regulate splicing. Previous work suggests that ced-4 can be alternatively spliced and that the splice variants function oppositely, with the longer transcript (ced-4L) inhibiting programmed cell death. spk-1 might promote cell survival by increasing the amount of the protective ced-4L splice variant. We conclude that programmed cell death in C. elegans is regulated by an alternative splicing event controlled by the SR protein kinase SPK-1.
|
巻・号 |
108(5)
|
ページ |
1998-2003
|
公開日 |
2011-2-1
|
DOI |
10.1073/pnas.1018805108
|
PII |
1018805108
|
PMID |
21245325
|
PMC |
PMC3033281
|
MeSH |
Alleles
Alternative Splicing
Animals
Apoptosis*
Caenorhabditis elegans / cytology
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Calcium-Binding Proteins / genetics
Genes, Helminth
Models, Genetic
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
Suppression, Genetic
|
IF |
9.412
|
引用数 |
8
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
線虫 |
tm837
tm722 |