論文 - 詳細
| RRC ID | 46593 |
|---|---|
| 著者 | Gleason JE, Eisenmann DM. |
| タイトル | Wnt signaling controls the stem cell-like asymmetric division of the epithelial seam cells during C. elegans larval development. |
| ジャーナル | Dev Biol |
| Abstract |
Metazoan stem cells repopulate tissues during adult life by dividing asymmetrically to generate another stem cell and a cell that terminally differentiates. Wnt signaling regulates the division pattern of stem cells in flies and vertebrates. While the short-lived nematode C. elegans has no adult somatic stem cells, the lateral epithelial seam cells divide in a stem cell-like manner in each larval stage, usually generating a posterior daughter that retains the seam cell fate and an anterior daughter that terminally differentiates. We show that while wild-type adult animals have 16 seam cells per side, animals with reduced function of the TCF homolog POP-1 have as many as 67 seam cells, and animals with reduced function of the β-catenins SYS-1 and WRM-1 have as few as three. Analysis of seam cell division patterns showed alterations in their stem cell-like divisions in the L2-L4 stages: reduced Wnt signaling caused both daughters to adopt non-seam fates, while activated Wnt signaling caused both daughters to adopt the seam fate. Therefore, our results indicate that Wnt signaling globally regulates the asymmetric, stem cell-like division of most or all somatic seam cells during C. elegans larval development, and that Wnt pathway regulation of stem cell-like behavior is conserved in nematodes. |
| 巻・号 | 348(1) |
| ページ | 58-66 |
| 公開日 | 2010-12-1 |
| DOI | 10.1016/j.ydbio.2010.09.005 |
| PII | S0012-1606(10)01055-9 |
| PMID | 20849842 |
| PMC | PMC2976807 |
| MeSH | Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Count Cell Differentiation Cell Division Cytoskeletal Proteins / deficiency Cytoskeletal Proteins / genetics Cytoskeletal Proteins / physiology* DNA-Binding Proteins / antagonists & inhibitors DNA-Binding Proteins / deficiency DNA-Binding Proteins / genetics DNA-Binding Proteins / physiology* Epithelial Cells / cytology* Epithelial Cells / metabolism Genes, Reporter High Mobility Group Proteins / antagonists & inhibitors High Mobility Group Proteins / deficiency High Mobility Group Proteins / genetics High Mobility Group Proteins / physiology* Larva RNA Interference Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / physiology Repressor Proteins / physiology Signal Transduction / genetics Signal Transduction / physiology* Transcription Factors / antagonists & inhibitors Transcription Factors / genetics Transcription Factors / physiology* Wnt Proteins / physiology* |
| IF | 2.896 |
| 引用数 | 37 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1883 |