RRC ID 46593
Author Gleason JE, Eisenmann DM.
Title Wnt signaling controls the stem cell-like asymmetric division of the epithelial seam cells during C. elegans larval development.
Journal Dev Biol
Abstract Metazoan stem cells repopulate tissues during adult life by dividing asymmetrically to generate another stem cell and a cell that terminally differentiates. Wnt signaling regulates the division pattern of stem cells in flies and vertebrates. While the short-lived nematode C. elegans has no adult somatic stem cells, the lateral epithelial seam cells divide in a stem cell-like manner in each larval stage, usually generating a posterior daughter that retains the seam cell fate and an anterior daughter that terminally differentiates. We show that while wild-type adult animals have 16 seam cells per side, animals with reduced function of the TCF homolog POP-1 have as many as 67 seam cells, and animals with reduced function of the β-catenins SYS-1 and WRM-1 have as few as three. Analysis of seam cell division patterns showed alterations in their stem cell-like divisions in the L2-L4 stages: reduced Wnt signaling caused both daughters to adopt non-seam fates, while activated Wnt signaling caused both daughters to adopt the seam fate. Therefore, our results indicate that Wnt signaling globally regulates the asymmetric, stem cell-like division of most or all somatic seam cells during C. elegans larval development, and that Wnt pathway regulation of stem cell-like behavior is conserved in nematodes.
Volume 348(1)
Pages 58-66
Published 2010-12-1
DOI 10.1016/j.ydbio.2010.09.005
PII S0012-1606(10)01055-9
PMID 20849842
PMC PMC2976807
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Count Cell Differentiation Cell Division Cytoskeletal Proteins / deficiency Cytoskeletal Proteins / genetics Cytoskeletal Proteins / physiology* DNA-Binding Proteins / antagonists & inhibitors DNA-Binding Proteins / deficiency DNA-Binding Proteins / genetics DNA-Binding Proteins / physiology* Epithelial Cells / cytology* Epithelial Cells / metabolism Genes, Reporter High Mobility Group Proteins / antagonists & inhibitors High Mobility Group Proteins / deficiency High Mobility Group Proteins / genetics High Mobility Group Proteins / physiology* Larva RNA Interference Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / physiology Repressor Proteins / physiology Signal Transduction / genetics Signal Transduction / physiology* Transcription Factors / antagonists & inhibitors Transcription Factors / genetics Transcription Factors / physiology* Wnt Proteins / physiology*
IF 2.896
Times Cited 37
C.elegans tm1883