RRC ID |
465
|
著者 |
Ota A, Kusakabe T, Sugimoto Y, Takahashi M, Nakajima Y, Kawaguchi Y, Koga K.
|
タイトル |
Cloning and characterization of testis-specific tektin in Bombyx mori.
|
ジャーナル |
Comp Biochem Physiol B Biochem Mol Biol
|
Abstract |
A testis-specific cDNA library of Bombyx mori was constructed by an mRNA subtraction technique. Several clones were randomly selected and determined for their nucleotide sequences. One of them, designated as BmTST, contained a 3'-part of an open reading frame homologous to tektin, the protein known to form filamentous polymers in the walls of ciliary and flagellar microtubules. Also isolated was a genomic fragment, which contains the 5'-part of the coding sequence of BmTST and its promoter region. As a whole, the complete open reading frame was found to encode 508 amino acid residues, whose sequence had 28, 28 and 30% identities with the Strongylocentrotus purpuratus tektins A1, B1 and C1, respectively. Expression analysis by reverse transcription polymerase chain reaction with the cDNA and Western blotting with a polyclonal antibody indicated that the BmTST gene was expressed specifically in the testis during sperm maturation. The protein was immunologically detected exclusively in the fraction expected to contain the 9 + 2 flagellar axonemes of sperms. We infer that the BmTst protein is possibly involved in the spermatogenesis of B. mori.
|
巻・号 |
133(3)
|
ページ |
371-82
|
公開日 |
2002-11-1
|
DOI |
10.1016/s1096-4959(02)00153-7
|
PII |
S1096495902001537
|
PMID |
12431405
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Bombyx / genetics*
Cloning, Molecular
DNA, Complementary / genetics
Gene Expression Profiling
Gene Library
Male
Microtubule Proteins / analysis
Microtubule Proteins / chemistry*
Microtubule Proteins / genetics*
Microtubules / chemistry
Molecular Sequence Data
Organ Specificity
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Spermatozoa / chemistry
Testis / cytology
Testis / metabolism*
|
IF |
2.219
|
引用数 |
23
|
WOS 分野
|
ZOOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
カイコ |
r06
EST database |