RRC ID 46604
著者 Harris G, Mills H, Wragg R, Hapiak V, Castelletto M, Korchnak A, Komuniecki RW.
タイトル The monoaminergic modulation of sensory-mediated aversive responses in Caenorhabditis elegans requires glutamatergic/peptidergic cotransmission.
ジャーナル J Neurosci
Abstract Monoamines and neuropeptides interact to modulate behavioral plasticity in both vertebrates and invertebrates. In Caenorhabditis elegans behavioral state or "mood" is dependent on food availability and is translated by both monoaminergic and peptidergic signaling in the fine-tuning of most behaviors. In the present study, we have examined the interaction of monoamines and peptides on C. elegans aversive behavior mediated by a pair of polymodal, nociceptive, ASH sensory neurons. Food or serotonin sensitize the ASHs and stimulate aversive responses through a pathway requiring the release of nlp-3-encoded neuropeptides from the ASHs. Peptides encoded by nlp-3 appear to stimulate ASH-mediated aversive behavior through the neuropeptide receptor-17 (NPR-17) receptor. nlp-3- and npr-17-null animals exhibit identical phenotypes and animals overexpressing either nlp-3 or npr-17 exhibit elevated aversive responses off food that are absent when nlp-3 or npr-17 are overexpressed in npr-17- or nlp-3-null animals, respectively. ASH-mediated aversive responses are increased by activating either Galpha(q) or Galpha(s) in the ASHs, with Galpha(s) signaling specifically stimulating the release of nlp-3-encoded peptides. In contrast, octopamine appears to inhibit 5-HT stimulation by activating Galpha(o) signaling in the ASHs that, in turn, inhibits both Galpha(s) and Galpha(q) signaling and the release of nlp-3-encoded peptides. These results demonstrate that serotonin and octopamine reversibly modulate the activity of the ASHs, and highlight the utility of the C. elegans model for defining interactions between monoamines and peptides in individual neurons of complex sensory-mediated circuits.
巻・号 30(23)
ページ 7889-99
公開日 2010-6-9
DOI 10.1523/JNEUROSCI.0497-10.2010
PII 30/23/7889
PMID 20534837
PMC PMC3005568
MeSH Animals Avoidance Learning / drug effects Avoidance Learning / physiology Biogenic Monoamines / metabolism Biogenic Monoamines / physiology* Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism GTP-Binding Protein alpha Subunits, Gs / metabolism Neuropeptides / metabolism* Nociceptors / drug effects Nociceptors / metabolism* Octanols / adverse effects Octopamine / pharmacology Serotonin / pharmacology Signal Transduction / drug effects*
IF 5.674
引用数 45
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm2302 tm2647 tm2654 tm3210 tm1652 tm3082 tm2974 tm2243 tm2765 tm1765 tm3202