RRC ID |
46604
|
著者 |
Harris G, Mills H, Wragg R, Hapiak V, Castelletto M, Korchnak A, Komuniecki RW.
|
タイトル |
The monoaminergic modulation of sensory-mediated aversive responses in Caenorhabditis elegans requires glutamatergic/peptidergic cotransmission.
|
ジャーナル |
J Neurosci
|
Abstract |
Monoamines and neuropeptides interact to modulate behavioral plasticity in both vertebrates and invertebrates. In Caenorhabditis elegans behavioral state or "mood" is dependent on food availability and is translated by both monoaminergic and peptidergic signaling in the fine-tuning of most behaviors. In the present study, we have examined the interaction of monoamines and peptides on C. elegans aversive behavior mediated by a pair of polymodal, nociceptive, ASH sensory neurons. Food or serotonin sensitize the ASHs and stimulate aversive responses through a pathway requiring the release of nlp-3-encoded neuropeptides from the ASHs. Peptides encoded by nlp-3 appear to stimulate ASH-mediated aversive behavior through the neuropeptide receptor-17 (NPR-17) receptor. nlp-3- and npr-17-null animals exhibit identical phenotypes and animals overexpressing either nlp-3 or npr-17 exhibit elevated aversive responses off food that are absent when nlp-3 or npr-17 are overexpressed in npr-17- or nlp-3-null animals, respectively. ASH-mediated aversive responses are increased by activating either Galpha(q) or Galpha(s) in the ASHs, with Galpha(s) signaling specifically stimulating the release of nlp-3-encoded peptides. In contrast, octopamine appears to inhibit 5-HT stimulation by activating Galpha(o) signaling in the ASHs that, in turn, inhibits both Galpha(s) and Galpha(q) signaling and the release of nlp-3-encoded peptides. These results demonstrate that serotonin and octopamine reversibly modulate the activity of the ASHs, and highlight the utility of the C. elegans model for defining interactions between monoamines and peptides in individual neurons of complex sensory-mediated circuits.
|
巻・号 |
30(23)
|
ページ |
7889-99
|
公開日 |
2010-6-9
|
DOI |
10.1523/JNEUROSCI.0497-10.2010
|
PII |
30/23/7889
|
PMID |
20534837
|
PMC |
PMC3005568
|
MeSH |
Animals
Avoidance Learning / drug effects
Avoidance Learning / physiology
Biogenic Monoamines / metabolism
Biogenic Monoamines / physiology*
Caenorhabditis elegans / cytology
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / metabolism*
GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism
GTP-Binding Protein alpha Subunits, Gs / metabolism
Neuropeptides / metabolism*
Nociceptors / drug effects
Nociceptors / metabolism*
Octanols / adverse effects
Octopamine / pharmacology
Serotonin / pharmacology
Signal Transduction / drug effects*
|
IF |
5.674
|
引用数 |
45
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
線虫 |
tm2302
tm2647
tm2654
tm3210
tm1652
tm3082
tm2974
tm2243
tm2765
tm1765
tm3202 |