論文 - 詳細
| RRC ID | 46616 |
|---|---|
| 著者 | Johnston WL, Krizus A, Dennis JW. |
| タイトル | Eggshell chitin and chitin-interacting proteins prevent polyspermy in C. elegans. |
| ジャーナル | Curr Biol |
| Abstract |
Development requires fertilization by a single sperm. In Caenorhabditis elegans, fertilization occurs in a sperm-filled spermatheca, implying the barrier to polyspermy is generated in this compartment. Eggshell chitin synthesis is initiated at fertilization, and chitin is deposited before the zygote exits the spermatheca. Whereas polyspermy is very rare in wild-type, here we report an incidence of 14%-51% in zygotes made chitin deficient by loss of chitin synthase-1 (CHS-1), the CHS-1 substrate UDP-N-acetylglucosamine, the CHS-1-interacting protein EGG-3, or the sperm-provided protein SPE-11. The spe-11(hc90) mutant deposits chitin at the male end but fails to complete a continuous layer. The polyspermy barrier is also compromised by loss of the chitin-binding protein CBD-1 or the GLD-1-regulated LDL receptor-like EGG-1, together with its homolog, EGG-2. Loss of CBD-1 or EGG-1/2 disrupts oocyte cortical distribution of CHS-1, as well as MBK-2 and EGG-3. In CBD-1 or EGG-1/2 deficiency, chitin is synthesized but the eggshell is fractured, suggesting aberrantly clustered CHS-1/MBK-2/EGG-3 may fail to support construction of a continuous eggshell. Together, our results show that eggshell chitin is required to prevent polyspermy in C. elegans, in addition to its previously reported requirement in polar body extrusion and polarization of the zygote. |
| 巻・号 | 20(21) |
| ページ | 1932-7 |
| 公開日 | 2010-11-9 |
| DOI | 10.1016/j.cub.2010.09.059 |
| PII | S0960-9822(10)01216-9 |
| PMID | 20971008 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Carrier Proteins / genetics Carrier Proteins / metabolism Carrier Proteins / physiology Chitin / genetics Chitin / physiology* Fertilization* / genetics Nuclear Proteins / genetics Nuclear Proteins / physiology Oocytes / metabolism Protein-Tyrosine Kinases / metabolism RNA Interference Zygote / metabolism |
| IF | 9.601 |
| 引用数 | 38 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1071 |