論文 - 詳細
| RRC ID | 46664 |
|---|---|
| 著者 | Pan X, Acar S, Scholey JM. |
| タイトル | Torque generation by one of the motor subunits of heterotrimeric kinesin-2. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Heterotrimeric kinesin-2 motors transport intraflagellar transport (IFT)-particles from the base to the tip of the axoneme to assemble and maintain cilia. These motors are distinct in containing two non-identical motor subunits together with an accessory subunit. We evaluated the significance of this organization by comparing purified wild type kinesin-2 holoenzymes that support IFT in vivo, with mutant trimers containing only one type of motor domain that do not support IFT in vivo. In motility assays, wild type kinesin-2 moved microtubules (MTs) at a rate intermediate between the rates supported by the two mutants. Interestingly, one of the mutants, but not the other mutant or the wild type protein, was observed to drive a persistent counter-clock-wise rotation of the gliding MTs. Thus one of the two motor domains of heterotrimeric kinesin-2 exerts torque as well as axial force as it moves along a MT, which may allow kinesin-2 to control its circumferential position around a MT doublet within the cilium. |
| 巻・号 | 401(1) |
| ページ | 53-7 |
| 公開日 | 2010-10-8 |
| DOI | 10.1016/j.bbrc.2010.09.007 |
| PII | S0006-291X(10)01682-7 |
| PMID | 20833139 |
| PMC | PMC3787135 |
| MeSH | A Kinase Anchor Proteins / chemistry A Kinase Anchor Proteins / genetics A Kinase Anchor Proteins / metabolism Amino Acid Sequence Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Molecular Sequence Data Protein Multimerization Protein Structure, Tertiary Torque* |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm324 |