Reference - Detail
| RRC ID | 46696 |
|---|---|
| Author | Ye B, Rui Q, Wu Q, Wang D. |
| Title | Metallothioneins are required for formation of cross-adaptation response to neurobehavioral toxicity from lead and mercury exposure in nematodes. |
| Journal | PLoS One |
| Abstract |
Metallothioneins (MTs) are small, cysteine-rich polypeptides, but the role of MTs in inducing the formation of adaptive response is still largely unknown. We investigated the roles of metallothionein genes (mtl-1 and mtl-2) in the formation of cross-adaptation response to neurobehavioral toxicity from metal exposure in Caenorhabditis elegans. Pre-treatment with mild heat-shock at L2-larva stage effectively prevented the formation of the neurobehavioral defects and the activation of severe stress response in metal exposed nematodes at concentrations of 50 and 100 µM, but pre-treatment with mild heat-shock did not prevent the formation of neurobehavioral defects in 200 µM of metal exposed nematodes. During the formation of cross-adaptation response, the induction of mtl-1 and mtl-2 promoter activity and subsequent GFP gene expression were sharply increased in 50 µM or 100 µM of metal exposed Pmtl-1::GFP and Pmtl-2::GFP transgenic adult animals after mild heat-shock treatment compared with those treated with mild heat-shock or metal exposure alone. Moreover, after pre-treatment with mild heat-shock, no noticeable increase of locomotion behaviors could be observed in metal exposed mtl-1 or mtl-2 mutant nematodes compared to those without mild heat-shock pre-treatment. The defects of adaptive response to neurobehavioral toxicity induced by metal exposure formed in mtl-1 and mtl-2 mutants could be completely rescued by the expression of mtl-1 and mtl-2 with the aid of their native promoters. Furthermore, over-expression of MTL-1 and MTL-2 at the L2-larval stage significantly suppressed the toxicity on locomotion behaviors from metal exposure at all examined concentrations. Therefore, the normal formation of cross-adaptation response to neurobehavioral toxicity induced by metal exposure may need the enough accumulation of MTs protein in animal tissues. |
| Volume | 5(11) |
| Pages | e14052 |
| Published | 2010-11-18 |
| DOI | 10.1371/journal.pone.0014052 |
| PMID | 21124968 |
| PMC | PMC2987793 |
| MeSH | Adaptation, Physiological / genetics Adaptation, Physiological / physiology* Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / drug effects Genetic Complementation Test Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Heat-Shock Response / genetics Heat-Shock Response / physiology Hot Temperature Lead / toxicity* Mercuric Chloride / toxicity* Mercury / toxicity* Metallothionein / genetics Metallothionein / metabolism* Motor Activity / drug effects Motor Activity / physiology* Mutation Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.74 |
| Times Cited | 22 |
| WOS Category | TOXICOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| C.elegans | tm1770 |