論文 - 詳細
| RRC ID | 46701 |
|---|---|
| 著者 | Zhang C, Montgomery TA, Gabel HW, Fischer SE, Phillips CM, Fahlgren N, Sullivan CM, Carrington JC, Ruvkun G. |
| タイトル | mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In Caenorhabditis elegans, mutator (mut) class genes mediate siRNA-guided repression of transposons as well as exogenous RNAi, but their roles in endogenous RNA silencing pathways are not well-understood. To characterize the endogenous small RNAs dependent on mut class genes, small RNA populations from a null allele of mut-16 as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi were subjected to deep sequencing. Additionally, each of the mut class genes was tested for a requirement in 26G siRNA pathways. The results indicate that mut-16 is an essential factor in multiple endogenous germline and somatic siRNA pathways involving several distinct Argonautes and RNA-dependent RNA polymerases. The results also reveal essential roles for mut-2 and mut-7 in the ERGO-1 class 26G siRNA pathway and less critical roles for mut-8, mut-14, and mut-15. We show that transposons are hypersusceptible to mut-16-dependent silencing and identify a requirement for the siRNA machinery in piRNA biogenesis from Tc1 transposons. We also show that the soma-specific mut-16(mg461) mutant allele is present in multiple C. elegans laboratory strains. |
| 巻・号 | 108(4) |
| ページ | 1201-8 |
| 公開日 | 2011-1-25 |
| DOI | 10.1073/pnas.1018695108 |
| PII | 1018695108 |
| PMID | 21245313 |
| PMC | PMC3029761 |
| MeSH | Alleles Animals Blotting, Northern Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* DNA Transposable Elements / genetics Embryo, Nonmammalian / metabolism Exoribonucleases / genetics Female Gene Expression Profiling Gene Expression Regulation, Developmental Germ Cells / metabolism Male Mutation RNA, Helminth / classification RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Small Interfering / classification RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / genetics* Species Specificity |
| IF | 9.412 |
| 引用数 | 56 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1661 tm1358 |