RRC ID 46701
Author Zhang C, Montgomery TA, Gabel HW, Fischer SE, Phillips CM, Fahlgren N, Sullivan CM, Carrington JC, Ruvkun G.
Title mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans.
Journal Proc Natl Acad Sci U S A
Abstract Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In Caenorhabditis elegans, mutator (mut) class genes mediate siRNA-guided repression of transposons as well as exogenous RNAi, but their roles in endogenous RNA silencing pathways are not well-understood. To characterize the endogenous small RNAs dependent on mut class genes, small RNA populations from a null allele of mut-16 as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi were subjected to deep sequencing. Additionally, each of the mut class genes was tested for a requirement in 26G siRNA pathways. The results indicate that mut-16 is an essential factor in multiple endogenous germline and somatic siRNA pathways involving several distinct Argonautes and RNA-dependent RNA polymerases. The results also reveal essential roles for mut-2 and mut-7 in the ERGO-1 class 26G siRNA pathway and less critical roles for mut-8, mut-14, and mut-15. We show that transposons are hypersusceptible to mut-16-dependent silencing and identify a requirement for the siRNA machinery in piRNA biogenesis from Tc1 transposons. We also show that the soma-specific mut-16(mg461) mutant allele is present in multiple C. elegans laboratory strains.
Volume 108(4)
Pages 1201-8
Published 2011-1-25
DOI 10.1073/pnas.1018695108
PII 1018695108
PMID 21245313
PMC PMC3029761
MeSH Alleles Animals Blotting, Northern Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* DNA Transposable Elements / genetics Embryo, Nonmammalian / metabolism Exoribonucleases / genetics Female Gene Expression Profiling Gene Expression Regulation, Developmental Germ Cells / metabolism Male Mutation RNA, Helminth / classification RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Small Interfering / classification RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / genetics* Species Specificity
IF 9.58
Times Cited 56
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm1661 tm1358