RRC ID 46703
著者 Zheng Q, Schaefer AM, Nonet ML.
タイトル Regulation of C. elegans presynaptic differentiation and neurite branching via a novel signaling pathway initiated by SAM-10.
ジャーナル Development
Abstract Little is known about transcriptional control of neurite branching or presynaptic differentiation, events that occur relatively late in neuronal development. Using the Caenorhabditis elegans mechanosensory circuit as an in vivo model, we show that SAM-10, an ortholog of mammalian single-stranded DNA-binding protein (SSDP), functions cell-autonomously in the nucleus to regulate synaptic differentiation, as well as positioning of, a single neurite branch. PLM mechanosensory neurons in sam-10 mutants exhibit abnormal placement of the neurite branch point, and defective synaptogenesis, characterized by an overextended synaptic varicosity, underdeveloped synaptic morphology and disrupted colocalization of active zone and synaptic vesicles. SAM-10 functions coordinately with Lim domain-binding protein 1 (LDB-1), demonstrated by our observations that: (1) mutations in either gene show similar defects in PLM neurons; and (2) LDB-1 is required for SAM-10 nuclear localization. SAM-10 regulates PLM synaptic differentiation by suppressing transcription of prk-2, which encodes an ortholog of the mammalian Pim kinase family. PRK-2-mediated activities of SAM-10 are specifically involved in PLM synaptic differentiation, but not other sam-10 phenotypes such as neurite branching. Thus, these data reveal a novel transcriptional signaling pathway that regulates neuronal specification of neurite branching and presynaptic differentiation.
巻・号 138(1)
ページ 87-96
公開日 2011-1-1
DOI 10.1242/dev.055350
PII dev.055350
PMID 21115607
PMC PMC2998167
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation / genetics Cell Differentiation / physiology* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Microscopy Neurites / metabolism* Reverse Transcriptase Polymerase Chain Reaction Synapses / metabolism
IF 5.611
引用数 9
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm674