RRC ID 46705
著者 Dejima K, Murata D, Mizuguchi S, Nomura KH, Izumikawa T, Kitagawa H, Gengyo-Ando K, Yoshina S, Ichimiya T, Nishihara S, Mitani S, Nomura K.
タイトル Two Golgi-resident 3'-Phosphoadenosine 5'-phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.
ジャーナル J Biol Chem
Abstract Synthesis of extracellular sulfated molecules requires active 3'-phosphoadenosine 5'-phosphosulfate (PAPS). For sulfation to occur, PAPS must pass through the Golgi membrane, which is facilitated by Golgi-resident PAPS transporters. Caenorhabditis elegans PAPS transporters are encoded by two genes, pst-1 and pst-2. Using the yeast heterologous expression system, we characterized PST-1 and PST-2 as PAPS transporters. We created deletion mutants to study the importance of PAPS transporter activity. The pst-1 deletion mutant exhibited defects in cuticle formation, post-embryonic seam cell development, vulval morphogenesis, cell migration, and embryogenesis. The pst-2 mutant exhibited a wild-type phenotype. The defects observed in the pst-1 mutant could be rescued by transgenic expression of pst-1 and hPAPST1 but not pst-2 or hPAPST2. Moreover, the phenotype of a pst-1;pst-2 double mutant were similar to those of the pst-1 single mutant, except that larval cuticle formation was more severely defected. Disaccharide analysis revealed that heparan sulfate from these mutants was undersulfated. Gene expression reporter analysis revealed that these PAPS transporters exhibited different tissue distributions and subcellular localizations. These data suggest that pst-1 and pst-2 play different physiological roles in heparan sulfate modification and development.
巻・号 285(32)
ページ 24717-28
公開日 2010-8-6
DOI 10.1074/jbc.M109.088229
PII S0021-9258(20)66338-4
PMID 20529843
PMC PMC2915708
MeSH Alleles Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / physiology* Gene Deletion Gene Expression Profiling Gene Expression Regulation, Developmental* Genes, Reporter Glycosaminoglycans / chemistry Golgi Apparatus / metabolism* Green Fluorescent Proteins / chemistry Heparitin Sulfate / metabolism* Mutation Nucleotide Transport Proteins / physiology* Subcellular Fractions Substrate Specificity Transgenes
IF 4.238
引用数 12
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm3364 tm3316