Reference - Detail
RRC ID | 46706 |
---|---|
Author | Imae R, Inoue T, Kimura M, Kanamori T, Tomioka NH, Kage-Nakadai E, Mitani S, Arai H. |
Title | Intracellular phospholipase A1 and acyltransferase, which are involved in Caenorhabditis elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol. |
Journal | Mol Biol Cell |
Abstract |
Phosphatidylinositol (PI), an important constituent of membranes, contains stearic acid as the major fatty acid at the sn-1 position. This fatty acid is thought to be incorporated into PI through fatty acid remodeling by sequential deacylation and reacylation. However, the genes responsible for the reaction are unknown, and consequently, the physiological significance of the sn-1 fatty acid remains to be elucidated. Here, we identified acl-8, -9, and -10, which are closely related to each other, and ipla-1 as strong candidates for genes involved in fatty acid remodeling at the sn-1 position of PI. In both ipla-1 mutants and acl-8 acl-9 acl-10 triple mutants of Caenorhabditis elegans, the stearic acid content of PI is reduced, and asymmetric division of stem cell-like epithelial cells is defective. The defects in asymmetric division of these mutants are suppressed by a mutation of the same genes involved in intracellular retrograde transport, suggesting that ipla-1 and acl genes act in the same pathway. IPLA-1 and ACL-10 have phospholipase A(1) and acyltransferase activity, respectively, both of which recognize the sn-1 position of PI as their substrate. We propose that the sn-1 fatty acid of PI is determined by ipla-1 and acl-8, -9, -10 and crucial for asymmetric divisions. |
Volume | 21(18) |
Pages | 3114-24 |
Published | 2010-9-15 |
DOI | 10.1091/mbc.E10-03-0195 |
PII | E10-03-0195 |
PMID | 20668164 |
PMC | PMC2938378 |
MeSH | Acyltransferases / genetics Acyltransferases / metabolism* Animals Animals, Genetically Modified Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division / physiology Fatty Acids / chemistry* Fatty Acids / metabolism HEK293 Cells Humans Mice Mutation Phosphatidylinositols / chemistry* Phosphatidylinositols / metabolism Phospholipases A1 / genetics Phospholipases A1 / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Stearic Acids / chemistry Stem Cells / cytology Stem Cells / physiology* |
IF | 3.791 |
Times Cited | 35 |
WOS Category | CELL BIOLOGY |
Resource | |
C.elegans | tm2290 tm1045 tm3232 tm3236 |