論文 - 詳細
| RRC ID | 4671 |
|---|---|
| 著者 | Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M. |
| タイトル | Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus. |
| ジャーナル | Mol Plant Microbe Interact |
| Abstract |
In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus. |
| 巻・号 | 21(4) |
| ページ | 375-82 |
| 公開日 | 2008-4-1 |
| DOI | 10.1094/MPMI-21-4-0375 |
| PMID | 18321183 |
| MeSH | Caulimovirus / genetics Gene Silencing* Genetic Vectors / genetics* Lotus / cytology Lotus / genetics* Models, Genetic Plant Leaves / genetics Plant Roots / genetics Plant Stems / genetics Plants, Genetically Modified Pollen / genetics Polyubiquitin / genetics* Promoter Regions, Genetic / genetics* Root Nodules, Plant / genetics |
| IF | 3.696 |
| 引用数 | 70 |
| WOS 分野 | PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Miyakojima MG-20 pUB vector |