RRC ID 4671
Author Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M.
Title Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus.
Journal Mol. Plant Microbe Interact.
Abstract In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
Volume 21(4)
Pages 375-82
Published 2008-4
DOI 10.1094/MPMI-21-4-0375
PMID 18321183
MeSH Caulimovirus / genetics Gene Silencing* Genetic Vectors / genetics* Lotus / cytology Lotus / genetics* Models, Genetic Plant Leaves / genetics Plant Roots / genetics Plant Stems / genetics Plants, Genetically Modified Pollen / genetics Polyubiquitin / genetics* Promoter Regions, Genetic / genetics* Root Nodules, Plant / genetics
IF 3.588
Times Cited 47
WOS Category PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Lotus / Glycine Miyakojima MG-20 pUB vector