RRC ID 46730
Author Le TT, Duren HM, Slipchenko MN, Hu CD, Cheng JX.
Title Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans.
Journal J Lipid Res
Abstract The ubiquity of lipids in biological structures and functions suggests that lipid metabolisms are highly regulated. However, current invasive techniques for lipid studies prevent characterization of the dynamic interactions between various lipid metabolism pathways. Here, we describe a noninvasive approach to study lipid metabolisms using a multifunctional coherent anti-Stokes Raman scattering (CARS) microscope. Using living Caenorhabditis elegans as a model organism, we report label-free visualization of coexisting neutral and autofluorescent lipid species. We find that the relative expression level of neutral and autofluorescent lipid species can be used to assay the genotype-phenotype relationship of mutant C. elegans with deletions in the genes encoding lipid synthesis transcription factors, LDL receptors, transforming growth factor beta receptors, lipid desaturation enzymes, and antioxidant enzymes. Furthermore, by coupling CARS with fingerprint confocal Raman analysis, we analyze the unsaturation level of lipids in wild-type and mutant C. elegans. Our study shows that complex genotype-phenotype relationships between lipid storage, peroxidation, and desaturation can be rapidly and quantitatively analyzed in a single living C. elegans.
Volume 51(3)
Pages 672-7
Published 2010-3-1
DOI 10.1194/jlr.D000638
PII S0022-2275(20)30525-3
PMID 19776402
PMC PMC2817597
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / physiology Gene Expression Regulation Lipid Metabolism* Lipid Peroxidation Microscopy, Fluorescence Mutation Spectrum Analysis, Raman / methods* Staining and Labeling
IF 4.483
Times Cited 82
C.elegans tm420 tm331 tm776