RRC ID 46895
著者 Tateno H, Yabe R, Sato T, Shibazaki A, Shikanai T, Gonoi T, Narimatsu H, Hirabayashi J.
タイトル Human ZG16p recognizes pathogenic fungi through non-self polyvalent mannose in the digestive system.
ジャーナル Glycobiology
Abstract Human zymogen granule protein 16 (ZG16p) contains a Jacalin-like lectin domain, although its glycan-binding properties are not fully understood. Here, we screened the glycan-binding specificity of ZG16p by recently developed glycoconjugate microarray. ZG16p appeared to exhibit selective binding to α- and β-linked mannose-polyacrylamide-biotin probes. In more quantitative analysis using frontal affinity chromatography, dissociation constants to two types of polyvalent mannose, i.e. high-density mannose and yeast mannan, were determined to be 1.3 and 1.7 µM, respectively. Mutation of the evolutionarily conserved amino acid Asp151, which is involved in sugar binding among the Jacalin-related lectins (JRLs), abolished binding activity to mannose. By immunohistochemical staining, ZG16p was specifically detected in mucus-secreting cells of the digestive system such as serosanguineous acinar cells of the parotid gland, acinar cells of the pancreas and goblet cells of the intestine. Finally, we showed that ZG16p recognizes pathogenic Candida and Malassezia species in a polyvalent mannose-dependent manner. We propose that ZG16p is a novel member of mannose-specific JRLs, which recognizes pathogenic fungi through non-self polyvalent mannose in the digestive system.
巻・号 22(2)
ページ 210-20
公開日 2012-2-1
DOI 10.1093/glycob/cwr130
PII cwr130
PMID 21893569
MeSH Binding Sites Candida / metabolism* Candida / pathogenicity Chromatography, Affinity / methods Humans Intestinal Mucosa / metabolism Intestines / chemistry Lectins / genetics Lectins / metabolism* Malassezia / metabolism* Malassezia / pathogenicity Mannose / chemistry Mannose / metabolism* Microarray Analysis / methods Mutation / genetics Pancreas / chemistry Pancreas / metabolism* Parotid Gland / chemistry Parotid Gland / metabolism* Protein Structure, Tertiary / genetics Recombinant Proteins / genetics Recombinant Proteins / metabolism Tissue Distribution
IF 4.06
引用数 24
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
病原微生物