Reference - Detail
| RRC ID | 47083 |
|---|---|
| Author | Kato K, Dong B, Wada H, Tanaka-Matakatsu M, Yagi Y, Hayashi S. |
| Title | Microtubule-dependent balanced cell contraction and luminal-matrix modification accelerate epithelial tube fusion. |
| Journal | Nat Commun |
| Abstract |
Connection of tubules into larger networks is the key process for the development of circulatory systems. In Drosophila development, tip cells of the tracheal system lead the migration of each branch and connect tubules by adhering to each other and simultaneously changing into a torus-shape. We show that as adhesion sites form between fusion cells, myosin and microtubules form polarized bundles that connect the new adhesion site to the cells' microtubule-organizing centres, and that E-cadherin and retrograde recycling endosomes are preferentially deposited at the new adhesion site. We demonstrate that microtubules help balancing tip cell contraction, which is driven by myosin, and is required for adhesion and tube fusion. We also show that retrograde recycling and directed secretion of a specific matrix protein into the fusion-cell interface promote fusion. We propose that microtubule bundles connecting these cell-cell interfaces coordinate cell contractility and apical secretion to facilitate tube fusion. |
| Volume | 7 |
| Pages | 11141 |
| Published | 2016-4-12 |
| DOI | 10.1038/ncomms11141 |
| PII | ncomms11141 |
| PMID | 27067650 |
| PMC | PMC4832058 |
| MeSH | Actins / metabolism Animals Biomechanical Phenomena Cadherins / metabolism Cell Fusion Cell Movement Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism Epithelial Cells / cytology* Extracellular Matrix / metabolism* Green Fluorescent Proteins / metabolism Intercellular Junctions / metabolism Microtubules / metabolism* Mutation / genetics Myosins / metabolism Phenotype Protein Transport Recombinant Fusion Proteins / metabolism Trachea / cytology |
| IF | 12.121 |
| Times Cited | 7 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 3327R-2 DGRC#119599 DGRC#119600 DGRC#119637 DGRC#119638 |