RRC ID 4718
Author Arunkumar KP, Tomar A, Daimon T, Shimada T, Nagaraju J.
Title WildSilkbase: an EST database of wild silkmoths.
Journal BMC Genomics
Abstract BACKGROUND:Functional genomics has particular promise in silkworm biology for identifying genes involved in a variety of biological functions that include: synthesis and secretion of silk, sex determination pathways, insect-pathogen interactions, chorionogenesis, molecular clocks. Wild silkmoths have hardly been the subject of detailed scientific investigations, owing largely to non-availability of molecular and genetic data on these species. As a first step, in the present study we generated large scale expressed sequence tags (EST) in three economically important species of wild silkmoths. In order to make these resources available for the use of global scientific community, an EST database called 'WildSilkbase' was developed.
DESCRIPTION:WildSilkbase is a catalogue of ESTs generated from several tissues at different developmental stages of 3 economically important saturniid silkmoths, an Indian golden silkmoth, Antheraea assama, an Indian tropical tasar silkmoth, A. mylitta and eri silkmoth, Samia cynthia ricini. Currently the database is provided with 57,113 ESTs which are clustered and assembled into 4,019 contigs and 10,019 singletons. Data can be browsed and downloaded using a standard web browser. Users can search the database either by BLAST query, keywords or Gene Ontology query. There are options to carry out searches for species, tissue and developmental stage specific ESTs in BLAST page. Other features of the WildSilkbase include cSNP discovery, GO viewer, homologue finder, SSR finder and links to all other related databases. The WildSilkbase is freely available from http://www.cdfd.org.in/wildsilkbase/.
CONCLUSION:A total of 14,038 putative unigenes was identified in 3 species of wild silkmoths. These genes provide important resources to gain insight into the functional and evolutionary study of wild silkmoths. We believe that WildSilkbase will be extremely useful for all those researchers working in the areas of comparative genomics, functional genomics and molecular evolution in general, and gene discovery, gene organization, transposable elements and genome variability of insect species in particular.
Volume 9
Pages 338
Published 2008-7-17
DOI 10.1186/1471-2164-9-338
PII 1471-2164-9-338
PMID 18637161
PMC PMC2483293
MeSH Animals Base Sequence Bombyx / genetics* Cluster Analysis Contig Mapping DNA, Complementary Databases, Genetic* Expressed Sequence Tags* Genes Genomics / methods Information Storage and Retrieval Internet Models, Genetic Molecular Sequence Data Polymorphism, Single Nucleotide Sequence Analysis, DNA Species Specificity User-Computer Interface
IF 3.594
Times Cited 34
Silkworms Samia cynthia ricini