| RRC ID |
4721
|
| 著者 |
Sato K, Matsunaga TM, Futahashi R, Kojima T, Mita K, Banno Y, Fujiwara H.
|
| タイトル |
Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis.
|
| ジャーナル |
Genetics
|
| Abstract |
Mutations at the flügellos (fl) locus in Bombyx mori produce wingless pupae and moths because of the repressed response of wing discs to ecdysteroid. Four recessive fl alleles occurred spontaneously and were mapped at 13.0 of the silkworm genetic linkage group 10. By positional cloning, we confirmed that the gene responsible for fl is fringe (fng) encoding Fng glycosyltransferase, which is involved in regulating the Notch signaling pathway. In four different fl alleles, we detected a large deletion of the fng gene in fl(k) and nonsense mutations in fl, fl(o), and fl(n). In the wild-type (WT) silkworm, fng is expressed actively in the wing discs, brain, and reproductive organs from the fourth to final instars but barely in the other tissues tested. In situ hybridization showed that fng mRNA is expressed in the dorsal layer of the WT wing discs. The wingless (wg) mRNA, a downstream marker of Fng-mediated Notch signaling, is localized at the dorsoventral boundary in the WT wing discs but repressed markedly in the fl wing discs. Although null mutants of Drosophila fng result in postembryonic lethality, loss of fng function in Bombyx affects only wing morphogenesis, suggesting different essential roles for fng in tissue differentiation among insects.
|
| 巻・号 |
179(2)
|
| ページ |
875-85
|
| 公開日 |
2008-6-1
|
| DOI |
10.1534/genetics.107.082784
|
| PII |
genetics.107.082784
|
| 解説 |
fl(無翅)の原因遺伝子はグリコシル転移酵素をコードするfngである。u03系統（fl/fl)では、fngのエクソン3に103bpの欠失が存在する。u04系統8flk/flk)では、fngの5’側を含んだ300kb以上の大きな欠失が存在する。u05系統(fln/fln)では、fngのエクソン2と3が重複している。
|
| PMID |
18505883
|
| PMC |
PMC2429881
|
| MeSH |
Alleles
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Bombyx / growth & development*
Bombyx / metabolism
Chromosomes, Artificial, Bacterial / genetics
Cloning, Molecular
Codon, Nonsense
DNA Primers / genetics
DNA, Complementary / genetics
Drosophila / genetics
Drosophila / growth & development
Gene Deletion
Gene Expression Profiling
Genes, Insect*
Insect Proteins / genetics
Larva / growth & development
Larva / metabolism
Molecular Sequence Data
Mutation*
Phylogeny
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Signal Transduction
Species Specificity
Tissue Distribution
Wings, Animal / growth & development*
Wings, Animal / metabolism
|
| IF |
4.015
|
| 引用数 |
23
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| カイコ |
u03
u04
u05 |
