RRC ID 47238
Author Imada K, Nakamura T.
Title The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast.
Journal Mol Biol Cell
Abstract During fission yeast sporulation, a membrane compartment called the forespore membrane (FSM) is newly formed on the spindle pole body (SPB). The FSM expands by membrane vesicle fusion, encapsulates the daughter nucleus resulting from meiosis, and eventually matures into the plasma membrane of the spore. Although many of the genes involved in FSM formation have been identified, its molecular mechanism is not fully understood. Here a genetic screen for sporulation-deficient mutations identified Ypt3, a Rab-family small GTPase known to function in the exocytic pathway. The ypt3-ki8 mutant showed defects in both the initiation of FSM biogenesis and FSM expansion. We also show that a mutation in Ypt2, another Rab protein that may function in the same pathway as Ypt3, compromises the initiation of FSM formation. As meiosis proceeds, both GFP-Ypt3 and GFP-Ypt2 are observed at the SPB and then relocalize to the FSM. Their localizations at the SPB precede FSM formation and depend on the meiotic SPB component Spo13, a putative GDP/GTP exchange factor for Ypt2. Given that Spo13 is essential for initiating FSM formation, these results suggest that two exocytic Rabs, Ypt3 and Ypt2, regulate the initiation of FSM formation on the SPB in concert with Spo13.
Volume 27(21)
Pages 3317-3328
Published 2016-11-1
DOI 10.1091/mbc.E16-03-0162
PII mbc.E16-03-0162
PMID 27630265
PMC PMC5170864
MeSH Cell Membrane / metabolism Cell Membrane / physiology Cell Nucleus / metabolism Gene Expression Regulation, Fungal Meiosis Membrane Fusion Protein Transport Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / metabolism Spindle Pole Bodies / metabolism Spores, Fungal / metabolism* rab GTP-Binding Proteins / metabolism*
IF 3.791
Times Cited 2
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