RRC ID 47245
Author Hisano H, Tsujimura M, Yoshida H, Terachi T, Sato K.
Title Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare).
Journal BMC Genomics
Abstract BACKGROUND:Sequencing analysis of mitochondrial genomes is important for understanding the evolution and genome structures of various plant species. Barley is a self-pollinated diploid plant with seven chromosomes comprising a large haploid genome of 5.1 Gbp. Wild barley (Hordeum vulgare ssp. spontaneum) and cultivated barley (H. vulgare ssp. vulgare) have cross compatibility and closely related genomes, although a significant number of nucleotide polymorphisms have been reported between their genomes.
RESULTS:We determined the complete nucleotide sequences of the mitochondrial genomes of wild and cultivated barley. Two independent circular maps of the 525,599 bp barley mitochondrial genome were constructed by de novo assembly of high-throughput sequencing reads of barley lines H602 and Haruna Nijo, with only three SNPs detected between haplotypes. These mitochondrial genomes contained 33 protein-coding genes, three ribosomal RNAs, 16 transfer RNAs, 188 new ORFs, six major repeat sequences and several types of transposable elements. Of the barley mitochondrial genome-encoded proteins, NAD6, NAD9 and RPS4 had unique structures among grass species.
CONCLUSIONS:The mitochondrial genome of barley was similar to those of other grass species in terms of gene content, but the configuration of the genes was highly differentiated from that of other grass species. Mitochondrial genome sequencing is essential for annotating the barley nuclear genome; our mitochondrial sequencing identified a significant number of fragmented mitochondrial sequences in the reported nuclear genome sequences. Little polymorphism was detected in the barley mitochondrial genome sequences, which should be explored further to elucidate the evolution of barley.
Volume 17(1)
Pages 824
Published 2016-10-24
DOI 10.1186/s12864-016-3159-3
PII 10.1186/s12864-016-3159-3
PMID 27776481
PMC PMC5078923
MeSH Base Sequence Computational Biology / methods DNA Transposable Elements Genome, Mitochondrial* Genome, Plant Genomics / methods High-Throughput Nucleotide Sequencing Hordeum / genetics* Molecular Sequence Annotation Open Reading Frames Phylogeny Polymorphism, Single Nucleotide Sequence Analysis, DNA
IF 3.501
Times Cited 13
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
Resource
Barley Haruna Nijo H602