RRC ID 47267
Author Minegishi Y, Sheng X, Yoshitake K, Sergeev Y, Iejima D, Shibagaki Y, Monma N, Ikeo K, Furuno M, Zhuang W, Liu Y, Rong W, Hattori S, Iwata T.
Title CCT2 Mutations Evoke Leber Congenital Amaurosis due to Chaperone Complex Instability.
Journal Sci Rep
Abstract Leber congenital amaurosis (LCA) is a hereditary early-onset retinal dystrophy that is accompanied by severe macular degeneration. In this study, novel compound heterozygous mutations were identified as LCA-causative in chaperonin-containing TCP-1, subunit 2 (CCT2), a gene that encodes the molecular chaperone protein, CCTβ. The zebrafish mutants of CCTβ are known to exhibit the eye phenotype while its mutation and association with human disease have been unknown. The CCT proteins (CCT α-θ) forms ring complex for its chaperon function. The LCA mutants of CCTβ, T400P and R516H, are biochemically instable and the affinity for the adjacent subunit, CCTγ, was affected distinctly in both mutants. The patient-derived induced pluripotent stem cells (iPSCs), carrying these CCTβ mutants, were less proliferative than the control iPSCs. Decreased proliferation under Cct2 knockdown in 661W cells was significantly rescued by wild-type CCTβ expression. However, the expression of T400P and R516H didn't exhibit the significant effect. In mouse retina, both CCTβ and CCTγ are expressed in the retinal ganglion cells and connecting cilium of photoreceptor cells. The Cct2 knockdown decreased its major client protein, transducing β1 (Gβ1). Here we report the novel LCA mutations in CCTβ and the impact of chaperon disability by these mutations in cellular biology.
Volume 6
Pages 33742
Published 2016-9-20
DOI 10.1038/srep33742
PII srep33742
PMID 27645772
PMC PMC5028737
MeSH Animals Cell Proliferation / genetics* Chaperonin Containing TCP-1* / genetics Chaperonin Containing TCP-1* / metabolism HEK293 Cells Humans Induced Pluripotent Stem Cells* / metabolism Induced Pluripotent Stem Cells* / pathology Leber Congenital Amaurosis* / genetics Leber Congenital Amaurosis* / metabolism Leber Congenital Amaurosis* / pathology Mutation* Protein Stability Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 3.998
Times Cited 12
DNA material pEF-BOS-FLAG-CCT beta (RDB15073) pEF-BOS-FLAG -T400P (RDB15074) pEF-BOS-FLAG-R516H (RDB15075) CSII-CMV-MCS-IRES2-Bsd (RDB04385)