RRC ID 4728
Author Chieda Y, Iiyama K, Lee JM, Kusakabe T, Yasunaga-Aoki C, Shimizu S.
Title Inactivation of pyocyanin synthesis genes has no effect on the virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori.
Journal FEMS Microbiol. Lett.
Abstract The contribution of pyocyanin to the virulence of Pseudomonas aeruginosa against the silkworm Bombyx mori was studied. First, purified pyocyanin was injected into the hemocoel of B. mori. Acute toxicity was observed only when a high dose of pyocyanin was injected. The lethal dose 50% value of pyocyanin was found to be 9.52 microg per larva. Next, mutant strains of phzM and phzS, which encode putative phenazine-specific methytransferase and flavin-containing monooxygenase, respectively, were created, and their virulence was compared with that of the PAO1 parent strain. Although the ability to produce pyocyanin was completely lost in the phz-mutant strains, they maintained the same level of virulence as the PAO1 parent strain. In addition, the complementation of the corresponding gene in trans in the mutant strains did not have any effect on the virulence of those mutant strains. These results indicated that pyocyanin does not act as a virulence factor in B. mori after invasion, which was different from the results obtained in other Lepidopteran host models.
Volume 278(1)
Pages 101-7
Published 2008-1
DOI 10.1111/j.1574-6968.2007.00982.x
PII FML982
PMID 18031534
MeSH Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism Bombyx / microbiology* Disease Models, Animal Host-Pathogen Interactions / genetics Larva Methyltransferases / genetics Methyltransferases / metabolism Mixed Function Oxygenases / genetics Mixed Function Oxygenases / metabolism Mortality Mutagenesis Pseudomonas aeruginosa / genetics* Pseudomonas aeruginosa / metabolism Pseudomonas aeruginosa / pathogenicity* Pyocyanine / biosynthesis Virulence Virulence Factors / genetics
IF 1.735
Times Cited 7
WOS Category MICROBIOLOGY
Resource
Silkworms ?