RRC ID 47280
Author Imai KS, Hudson C, Oda-Ishii I, Yasuo H, Satou Y.
Title Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos.
Journal Development
Abstract Many animal embryos use nuclear β-catenin (nβ-catenin) during the segregation of endomesoderm (or endoderm) from ectoderm. This mechanism is thus likely to be evolutionarily ancient. In the ascidian embryo, nβ-catenin reiteratively drives binary fate decisions between ectoderm and endomesoderm at the 16-cell stage, and then between endoderm and margin (mesoderm and caudal neural) at the 32-cell stage. At the 16-cell stage, nβ-catenin activates endomesoderm genes in the vegetal hemisphere. At the same time, nβ-catenin suppresses the DNA-binding activity of a maternal transcription factor, Gata.a, through a physical interaction, and Gata.a thereby activates its target genes only in the ectodermal lineage. In the present study, we found that this antagonism between nβ-catenin and Gata.a also operates during the binary fate switch at the 32-cell stage. Namely, in marginal cells where nβ-catenin is absent, Gata.a directly activates its target, Zic-r.b (ZicL), to specify the marginal cell lineages. Thus, the antagonistic action between nβ-catenin and Gata.a is involved in two consecutive stages of germ layer segregation in ascidian embryos.
Volume 143(22)
Pages 4167-4172
Published 2016-11-15
DOI 10.1242/dev.141481
PII dev.141481
PMID 27707797
MeSH Animals Animals, Genetically Modified Body Patterning / genetics* Cell Lineage / genetics Ciona intestinalis / embryology* Ciona intestinalis / genetics Embryo, Nonmammalian GATA1 Transcription Factor / antagonists & inhibitors* GATA1 Transcription Factor / genetics Gene Expression Regulation, Developmental Germ Layers / cytology Germ Layers / embryology* Germ Layers / metabolism Urochordata / embryology Urochordata / genetics beta Catenin / antagonists & inhibitors* beta Catenin / genetics
IF 5.763
Times Cited 0
WOS Category DEVELOPMENTAL BIOLOGY
Resource
C.intestinalis / (O.japonicus) Wild C. int