RRC ID 4742
Author Hoshino A, Park KI, Iida S.
Title Identification of r mutations conferring white flowers in the Japanese morning glory (Ipomoea nil).
Journal J Plant Res
Abstract The wild-type Japanese morning glory [Ipomoea nil (L.) Roth.] exhibits blue flowers with red stems, and spontaneous r mutants display white flowers with green stems. We have identified two r mutations, r1-1 and r1-2, that are caused by insertions of Tpn1-related DNA transposable elements, Tpn3 (5.6 kb) and Tpn6 (4.7 kb), respectively, into a unique intron of the CHS-D gene, which is responsible for flower and stem pigmentation. Both Tpn3 and Tpn6, which belong to the En/Spm or CACTA superfamily, are nonautonomous elements lacking transposase genes but containing unrelated cellular DNA segments including exons and introns. Interestingly, r1-2 contains an additional 4-bp insertion at the Tpn3 integration site in r1-1, presumably a footprint caused by the excision of Tpn3. The results strengthen the previous notion that Tpn1 and its relatives are major spontaneous mutagens for generating various floriculturally important traits in I. nil. Since I. nil has an extensive history of genetic studies, molecular identification of classical spontaneous mutations would also facilitate reinterpretation of the abundant classical genetic data available.
Volume 122(2)
Pages 215-22
Published 2009-3-1
DOI 10.1007/s10265-008-0202-8
PMID 19085046
MeSH Anthocyanins / biosynthesis Base Sequence DNA, Plant / chemistry Flowers / genetics* Flowers / metabolism Genes, Plant Ipomoea nil / genetics* Ipomoea nil / metabolism Models, Genetic Molecular Sequence Data Mutation* Pigmentation / genetics* Plant Proteins / genetics Plant Proteins / metabolism
IF 2.082
Times Cited 15
Morning Glory AK11 AK14 AK15 AK16 AK17 AK18 AK19 AK20 AK21 AK22