RRC ID 47433
Author Miyake Y, Nakamura M, Nabetani A, Shimamura S, Tamura M, Yonehara S, Saito M, Ishikawa F.
Title RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway.
Journal Mol Cell
Abstract Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins contain putative OB-fold domains and form a complex called CST, which binds to single-stranded DNA with high affinity in a sequence-independent manner. CST associates with a fraction of telomeres consistently during the cell cycle, in quiescent cells and Pot1-knockdown cells. It does not colocalize with replication foci in S phase. Significant increases in the abundance of single-stranded G-strand telomeric DNA were observed in Stn1-knockdown cells. We propose that CST is a replication protein A (RPA)-like complex that is not directly involved in conventional DNA replication at forks but plays a role in DNA metabolism frequently required by telomeres.
Volume 36(2)
Pages 193-206
Published 2009-10-23
DOI 10.1016/j.molcel.2009.08.009
PII S1097-2765(09)00587-5
PMID 19854130
MeSH Animals Base Sequence DNA, Single-Stranded / metabolism* HeLa Cells Humans Mice Molecular Sequence Data Multiprotein Complexes / metabolism Mutant Proteins / metabolism Protein Binding Protein Multimerization Protein Structure, Tertiary Protein Transport Recombinant Proteins Replication Protein A / metabolism* Sequence Homology, Amino Acid Shelterin Complex Telomere / metabolism* Telomere-Binding Proteins / chemistry Telomere-Binding Proteins / metabolism*
IF 15.584
Times Cited 210
DNA material pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).